Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4

Mouse monoclonal IgG2a, kappa isotype control antibody
Catalog #
60108_C
Mouse monoclonal IgG2a, kappa isotype control antibody
From: 95 USD

Overview

The C1.18.4 antibody (IgG2a, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being employed. If a conjugated antibody is employed, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry.
Subtype
Isotype Controls
Target Antigen
IgG2a Isotype Control
Alternative Names
Not applicable
Conjugation
APC, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blotting
Area of Interest
Immunology
Clone
C1.18.4
Gene ID
Not applicable
Isotype
IgG2a, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4
Catalog #
60108
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC
Catalog #
60108AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC
Catalog #
60108FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE
Catalog #
60108PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5
Catalog #
60108PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4
Catalog #
60108
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC
Catalog #
60108AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC
Catalog #
60108FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE
Catalog #
60108PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5
Catalog #
60108PS
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Catalog #60097) followed by a rat anti-mouse IgG2a antibody, PE.

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, APC, Catalog #60097AZ).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, FITC, Catalog #60097FI).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PE, Catalog #60097PE).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5, Catalog #60097PS).

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