Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4

Mouse monoclonal IgG2a, kappa isotype control antibody

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Mouse monoclonal IgG2a, kappa isotype control antibody
From: 90 USD

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The C1.18.4 antibody (IgG2a, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being employed. If a conjugated antibody is employed, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry.
Subtype:
Isotype Controls
Target Antigen:
IgG2a Isotype Control
Alternative Names:
Not applicable
Conjugation:
APC; FITC; PE; Unconjugated; PerCP-Cyanine5.5
Host Species:
Mouse
Cell Type:
Other
Application:
Flow Cytometry; Functional Assay; Immunohistochemistry; Western Blotting
Area of Interest:
Immunology
Clone:
C1.18.4
Gene ID:
Not applicable
Isotype:
IgG2a, kappa

Scientific Resources

Product Documentation

Educational Materials

(3)
Brochure
Complete Antibody Listing
Brochure
Immunology Antibodies
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
CHO and Other Cell Line Development
Transfection and Selection
Cloning
Expansion and Characterization
Hybridoma Production
Pre-Enrichment and Fusion
Selection and Cloning
Expansion and Characterization
Mesenchymal Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Culture and Cryopreservation
Differentiation
Characterization

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Catalog #60097) followed by a rat anti-mouse IgG2a antibody, PE.

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, APC, Catalog #60097AZ).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, FITC, Catalog #60097FI).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PE, Catalog #60097PE).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5, Catalog #60097PS).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.