Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4
Overview
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry.
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Educational Materials (2)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Catalog #60097) followed by a rat anti-mouse IgG2a antibody, PE.
Figure 2. Data for APC-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, APC, Catalog #60097AZ).
Figure 3. Data for FITC-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, FITC, Catalog #60097FI).
Figure 4. Data for PE-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PE, Catalog #60097PE).
Figure 5. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5, Catalog #60097PS).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
