DNase I
For digestion of DNA
Overview
Deoxyribonuclease I (DNase I) is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Subtype
Enzymatic
Alternative Names
DNA endonuclease; DNA nuclease; Deoxyribonucleic phosphatase; Pancreatic DNase; Thymonuclease
Cell Type
B Cells, Endothelial Colony Forming Cells (ECFCs), Neurons, Osteoblasts, T Cells
Species
Human, Mouse, Rat, Non-Human Primate, Other
Area of Interest
Cancer, Endothelial Cell Biology, Epithelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
29.1 kDa
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
DNase I
|
Catalog #
07469, 07470, 100-0683 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet
|
Product Name
DNase I
|
Catalog #
07469, 07470, 100-0683 |
Lot #
All |
Language
English |
Educational Materials (1)
Wallchart
Human Immune Cytokines
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
B Cell Research
Workflow Stages for
Dendritic Cell Research
Workflow Stages for
Granulocyte Research
Workflow Stages for
Total Lymphocyte Research
Workflow Stages for
Mammary Epithelial Cell Research
Workflow Stages for
Monocyte Research
Workflow Stages for
Myeloid Cell Research
Workflow Stages for
Natural Killer Cell Research
Workflow Stages for
Prostate Epithelial Cell Research
Workflow Stages for
T Cell Research
Workflow Stages for
HLA Analysis
Workflow Stages for
Chimerism Analysis
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
