Dispase, ACF

Animal component-free non-specific protease
Catalog #
07446_C
Animal component-free non-specific protease
From: 232 USD

Overview

Dispase, Animal Component-Free (ACF) is a neutral, amino-endoprotease that cleaves the N-terminal peptide bond of non-polar amino acid residues and is obtained from cultures free of animal-derived materials. Dispase has mild proteolytic activity, which makes it especially useful for the isolation and passaging of primary cells. Its proteolytic activity also allows it to maintain membrane integrity. Dispase is commonly used with other proteases such as collagenase in cell isolation and tissue dissociation for neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).
Subtype
Enzymatic
Alternative Names
Neutral protease; Proteinase
Cell Type
Chondrocytes, Neural Stem and Progenitor Cells, Osteoblasts
Species
Human, Mouse, Rat, Non-Human Primate, Other
Area of Interest
Cancer Research, Endothelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
32.5 kDa

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Dispase, ACF
Catalog #
07446, 100-0396
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Dispase, ACF
Catalog #
07446, 100-0396
Lot #
All
Language
English

Educational Materials(1)

Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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