STEMdiff™ Neuron Differentiation Kit

Differentiation kit for generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells

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STEMdiff™ Neuron Differentiation Kit

Differentiation kit for generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells

1 Kit
Catalog #08500
225 USD

Required Products

Overview

The STEMdiff™ Neuron Differentiation Kit is used to generate neuronal precursors from neural progenitor cells derived from human pluripotent stem cells using the STEMdiff™ Neural Induction Medium (Catalog #05835) embryoid body protocol. The neuronal precursors generated can be matured using the STEMdiff™ Neuron Maturation Kit (Catalog #08510) to produce a mixed population of excitatory and inhibitory forebrain-type (FOXG1-positive) neurons (≥ 90% class III β-tubulin-positive neurons; < 1% GFAP-positive astrocytes). Cells derived using these products are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing and cell therapy validation.
Advantages:
• Defined and serum-free
• Supports highly efficient generation of neuronal precursors from hPSC-derived NPCs
• Precursors generated can be matured to a highly pure population of mixed neurons using the STEMdiff™ Neuron Maturation Kit
• Optimized for differentiation of NPCs generated using the STEMdiff™ Neural Induction Medium embryoid body protocol
• Enables reproducible differentiation of NPCs derived from multiple human ES and iPS cell lines
Components:
  • STEMdiff™ Neuron Differentiation Basal Medium, 100 mL
  • STEMdiff™ Neuron Differentiation Supplement A, 8 mL
  • STEMdiff™ Neuron Differentiation Supplement B, 2 mL
  • STEMdiff™ Neuron Differentiation Supplement C, 200 µL
Subtype:
Specialized Media
Cell Type:
Neural Cells, PSC-Derived; Neural Stem and Progenitor Cells; Neurons; Pluripotent Stem Cells
Species:
Human
Application:
Cell Culture; Differentiation; Toxicity Assay
Brand:
STEMdiff
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Neuroscience; Stem Cell Biology
Formulation:
Serum-Free

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Downstream Differentiation of Neural Progenitor Cells to Forebrain Neurons

(A-B) Neural Progenitor Cells derived from ES cells (H9 cell line) using the STEMdiff™ Neural Induction Medium embryoid body protocol were differentiated and matured to forebrain neurons by culture in STEMdiff™ Neuron Differentiation Kit for 7 days and STEMdiff™ Neuron Maturation Kit for 21 days. A mixed population of excitatory and inhibitory neurons was formed. The resulting cultures contain a highly pure population of class III β-tubulin-positive neurons (A-B, red), with a high proportion of GABAergic interneurons (A, GABA, green). The neurons generated are forebrain-type (B, FOXG1, green).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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