STEMdiff™ Neuron Differentiation Kit
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STEMdiff™ Neuron Differentiation Kit -
Label for STEMdiff™ Neuron Differentiation Kit -
Label for STEMdiff™ Neuron Differentiation Kit -
Label for STEMdiff™ Neuron Differentiation Kit -
Label for STEMdiff™ Neuron Differentiation Kit
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Required Products
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STEMdiff™ Neuron Maturation Kit
Maturation kit for generation of functional neurons from human ES and iPS cell-derived neuronal precursor cells
Overview
• Supports highly efficient generation of neuronal precursors from hPSC-derived NPCs
• Precursors generated can be matured to a highly pure population of mixed neurons using the STEMdiff™ Neuron Maturation Kit
• Optimized for differentiation of NPCs generated using the STEMdiff™ Neural Induction Medium embryoid body protocol
• Enables reproducible differentiation of NPCs derived from multiple human ES and iPS cell lines
- STEMdiff™ Neuron Differentiation Basal Medium, 100 mL
- STEMdiff™ Neuron Differentiation Supplement A, 8 mL
- STEMdiff™ Neuron Differentiation Supplement B, 2 mL
- STEMdiff™ Neuron Differentiation Supplement C, 200 µL
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STEMdiff™ Neural Induction Medium
Defined, serum-free medium for neural induction of human ES and iPS cells -
STEMdiff™ Neural Rosette Selection Reagent
Enzyme-free reagent for the selective detachment of neural rosettes
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Product Documentation
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Data and Publications
Data
Figure 1. Downstream Differentiation of Neural Progenitor Cells to Forebrain Neurons
(A-B) Neural Progenitor Cells derived from ES cells (H9 cell line) using the STEMdiff™ Neural Induction Medium embryoid body protocol were differentiated and matured to forebrain neurons by culture in STEMdiff™ Neuron Differentiation Kit for 7 days and STEMdiff™ Neuron Maturation Kit for 21 days. A mixed population of excitatory and inhibitory neurons was formed. The resulting cultures contain a highly pure population of class III β-tubulin-positive neurons (A-B, red), with a high proportion of GABAergic interneurons (A, GABA, green). The neurons generated are forebrain-type (B, FOXG1, green).