Fibroblast growth factor acidic (FGF-acidic), also known as FGF-1, is a potent activator of DNA synthesis, cell proliferation, and chemotaxis and is known to play numerous roles in development, regeneration, and angiogenesis (Galzie et al.; Jaye et al.; Presta et al.). FGF-acidic is produced by multiple cell types and is capable of activating all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It is found in large quantities in the brain, but is also expressed in hepatocytes, vascular smooth muscle cells, neurons of the central nervous system, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelial cells, and pituitary basophils and acidophils. FGF-acidic is secreted as a disulfide-linked homodimer and is stored in complex with heparan sulfate, a requirement for its interaction with FGF receptors (Guerrini et al.; Mohammadi et al.). Internalized FGF-acidic signals via protein kinase C and promotes cell survival by inhibiting p53 and proapoptotic signaling (Bouleau et al.). This product is animal component-free.
(A) The biological activity of Human Recombinant FGF-acidic, ACF was tested by its ability to promote proliferation of NR6R-3T3 cells in
the presence of 10 μg/mL heparin. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the
effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.0277
(B) 1 μg of Human Recombinant FGF-acidic, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and
visualized by Coomassie Blue staining. Human Recombinant FGF-acidic, ACF has a predicted molecular mass of 16.0 kDa.
This product is designed for use in the following research area(s) as part
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For differentiation of human ES or iPS cells into hematopoietic progenitor cells
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Human Recombinant FGF-acidic, ACF
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