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IGF-I long arginine 3 (LR3) binds to insulin-like growth factor 1 (IGF-I) receptor and is a potent activator of the PI3K/AKT pathway and ERK1/2 signaling. IGF-I is a polypeptide that belongs to the family of insulin-like growth factors similar in molecular structure to proinsulin and is a synthetic analog of insulin-like growth factor 1 (IGF-I; Francis et al). IGF-I LR3 has >100-fold reduced affinity for IGF-binding proteins (IGFBPs), which reduces availability of IGFs in cell culture (Voorhamme & Yandell). IGF-I is required for embryonic development, and is produced mainly in the liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for human pluripotent stem cell maintenance (Wang et al.). Together with IL-3, IGF-I stimulates differentiation and proliferation of myeloid cells and has been shown to regulate lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs (Heemskerk et al.). This product is animal component-free (ACF).
Subtype
Cytokines
Alternative Names
IBP1, IGF-IA, IGF-IB, IGF1A, Insulin-like growth factor 1, Long R3 IGF-I, Mechano growth factor, MGF, Somatomedin C
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Prostate Cells
Figure 1. Biological Activity and Molecular Mass of Human Recombinant IGF-I LR3, ACF
(A) The biological activity of Human Recombinant IGF-I LR3, ACF was tested by its ability to induce MAP/ERK signaling in transfected MCF-7 cells using a luciferase reporter assay. Firefly luciferase activity is normalized to the control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which IGF-I LR3 response is at 50% of maximum. The EC50 in the above example is 1.3 nM (11.7 ng/mL).
(B) 3 μg of Human Recombinant IGF-I LR3, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IGF-I LR3, ACF has a predicted molecular mass of 9 kDa.
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