STEMdiff™ Forebrain Neuron Maturation Kit
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STEMdiff™ Forebrain Neuron Maturation Kit -
Label for BrainPhys™ Neuronal Basal Medium -
Label for STEMdiff™ Forebrain Neuron Maturation Supplement
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Required Products
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STEMdiff™ Forebrain Neuron Differentiation Kit
Differentiation kit for the generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells -
STEMdiff™ SMADi Neural Induction Kit
Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells -
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer -
STEMdiff™ Neural Rosette Selection Reagent
Enzyme-free reagent for the selective detachment of neural rosettes
Overview
• Supports highly efficient generation of functional neurons from hPSC-derived neuronal precursors
• Produces a highly pure population (≥ 90% neurons) of mixed excitatory and inhibitory neurons that can be maintained long-term in culture
• Optimized for maturation of neuronal precursors generated using the STEMdiff™ Forebrain Neuron Differentiation Kit
• Supports neuronal activity for physiologically relevant results
• Enables reproducible maturation of neuronal precursors derived from multiple human ES and iPS cell lines
- BrainPhys™ Neuronal Medium
- STEMdiff™ Forebrain Neuron Maturation Supplement
Related Products
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NeuroCult™ SM1 Neuronal Supplement
Supplement for the serum-free culture of primary tissue-derived neurons and hPSC-derived neural progenitor cells -
Serum-free neurophysiological basal medium for improved neuronal function -
For neural and pancreatic differentiation of mouse and human ES and iPS cells -
Anti-Beta-Tubulin III Antibody, Clone TUJ1
Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III
Scientific Resources
Product Documentation
Educational Materials
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Schematic for the Embryoid Body Protocol
Forebrain-type neurons can be generated from neural precursors after 6 days in STEMdiff™ Forebrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.
Figure 2. Schematic for the Monolayer Protocol
Forebrain-type neurons can be generated from neural precursors after 6 days in STEMdiff™ Forebrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.
Figure 3. Forebrain-Type Neurons Are Generated After Culture in STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits
NPCs generated from hPSCs in mTeSR 1™ using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to forebrain-type neurons using the STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits. (A) Forebrain-type neurons were formed after iPS cell-derived NPCs were cultured with the STEMdiff™ Forebrain Neuron Differentiation Kit for 7 days and STEMdiff™ Forebrain Neuron Maturation Kit for 14 days. The resulting cultures contain a highly pure population of (B) class III β-tubulin-positive neurons (green), with (C) fewer than 10% astrocytes (GFAP-positive cells, red). (D) Nuclei are labeled with DAPI (blue).
Figure 4. Performance of STEMdiff™ Neuron and the STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits
Neural progenitor cells (NPCs) were derived using the monolayer protocol for the STEMdiff™ SMADi Neural Induction Kit, from H9 ES cells maintained in mTeSR™ 1. NPCs were then differentiated into forebrain-type neurons using either A) STEMdiff™ Neuron Differentiation and Maturation Kits or B) the equivalent-performing STEMdiff™ Forebrain Neuron Differentiation and Maturation Kits according to protocol instructions (see product information sheets for Catalog #08600/08605). The resulting neurons were fixed for immunofluorescence. Both sets of products demonstrate equivalent differentiation efficiency, with a yield of ≥90% class III ꞵ-tubulin-positive neurons (green) and ≤10% GFAP-positive astrocytes (red).