Mouse Recombinant IL-12

Interleukin 12
Catalog #
78028_C
Interleukin 12
From: 208 EUR

Overview

Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann & Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).
Subtype
Cytokines
Alternative Names
CLMF, CTL maturation factor, Cytotoxic lymphocyte maturation factor, IL-12p70, Interleukin-12, Natural killer cells stimulator factor, NKSF
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, Lymphocytes, NK Cells, T Cells
Species
Mouse
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
57.5 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Mouse Recombinant IL-12
Catalog #
78028.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Mouse Recombinant IL-12
Catalog #
78028.1
Lot #
All
Language
English

Educational Materials (3)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines

Data and Publications

Data

(A) The biological activity of Mouse Recombinant IL-12 was tested by its ability to induce SEAP in HEK-Blue™ IL-12 cells. The EC50 is defined as the effective concentration of the growth factor at which expression of CD25 is at 50% of maximum. The EC50 in the above example is 2.3 ng/mL. For a representative bioactivity plot of CD25 expression on CD8+ T cells, contact us at techsupport@stemcell.com.
(B) 1 μg of Mouse Recombinant IL-12 was resolved with SDS-PAGE under reducing (+) non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-12 is a heterodimer comprising one p40 subunit and one p35 subunit. The total predicted molecular mass is 57.5 kDa but migrates to an apparent molecular mass of 70 kDa.

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