IDE1

Activin/BMP/TGF-β pathway activator

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Activin/BMP/TGF-β pathway activator
From: 111 USD

Overview

Inducer of definitive endoderm 1 (IDE1) induces differentiation of mouse or human pluripotent stem cells (PSCs) by activating SMAD2 phosphorylation and NODAL expression (Borowiak et al.). At EC₅₀ = 125 nM, SOX17 expression was induced in mouse ES cells.

DIFFERENTIATION
· Induces differentiation of mouse or human ES cells to definitive endoderm in the absence of Activin A, NODAL, or feeder cells (Borowiak et al.).
Alternative Names:
Not applicable
CAS Number:
1160927-48-9
Chemical Formula:
C₁₅H₁₈N₂O₅
Molecular Weight:
306.3 g/mol
Purity:
≥ 95%
Pathway:
Activin/Nodal/TGFβ

Scientific Resources

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Educational Materials

(3)

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications

(1)
Cell stem cell 2009 APR

Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells.

Borowiak M et al.

Abstract

An essential step for therapeutic and research applications of stem cells is the ability to differentiate them into specific cell types. Endodermal cell derivatives, including lung, liver, and pancreas, are of interest for regenerative medicine, but efforts to produce these cells have been met with only modest success. In a screen of 4000 compounds, two cell-permeable small molecules were indentified that direct differentiation of ESCs into the endodermal lineage. These compounds induce nearly 80% of ESCs to form definitive endoderm, a higher efficiency than that achieved by Activin A or Nodal, commonly used protein inducers of endoderm. The chemically induced endoderm expresses multiple endodermal markers, can participate in normal development when injected into developing embryos, and can form pancreatic progenitors. The application of small molecules to differentiate mouse and human ESCs into endoderm represents a step toward achieving a reproducible and efficient production of desired ESC derivatives.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.