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Human Recombinant IL-4, ACF

Interleukin 4, animal component-free

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Human Recombinant IL-4, ACF

Interleukin 4, animal component-free

From: 227 USD
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Interleukin 4, animal component-free
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Overview

Human Interleukin 4 (IL-4) is important for immune responses to helminth infection as well as in allergic responses (Olpihant et al.). The IL-4 receptor consists of a heterodimer of IL-4Ra and common gamma chain. IL-4 receptor engagement leads to the activation of JAK1/3 and the recruitment of STAT6 and IRS1/2 (Nelms et al.). IL-4 drives immunoglobulin class switching in B cells (to IgE, IgG4), mast cell hyperplasia, mucus production, and the differentiation of naïve T cells into T helper type 2 (Th2) cells, which produce IL-4, IL-5, IL-10, and IL-13 (Bao et al.; Olpihant et al.; Nelms et al.). In addition to Th2 T cells, IL-4 is produced by CD4+ NK T cells, γ/δ T cells, activated basophils, eosinophils, and mast cells. IL-4 consists of 130 amino acids with a predicted molecular weight of 15.1 kDa. Human IL-4 does not cross-react with mouse cells (Park et al.). This product is animal component-free.
Subtype
Cytokines
Cell Type
B Cells, Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Lymphocytes, Monocytes, Myeloid Cells, Neurons, T Cells
Species
Human
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant IL-4, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.2 ng/mL. (B) 1 μg of Human Recombinant IL-4, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-4, ACF
Catalog #
78147, 78147.1, 78147.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-4, ACF
Catalog #
78147, 78147.1, 78147.2
Lot #
All
Language
English

Applications

Resources and Publications

Educational Materials (5)

Cytokines, Chemokines and Growth Factors
Brochure
Cytokines, Chemokines and Growth Factors
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct

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Quality Statement:

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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