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Data and Publications
(A) The biological activity of Human Recombinant IFN-gamma was tested by its ability to promote the cytotoxicity of HT-29 (HTB-38) cells.
Cytotoxicity was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at
which cell death is at 50% of maximum. The EC50 for this effect is 0.035 ng/mL.
(B) 2 μg of Human Recombinant IFN-gamma was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-gamma has a predicted molecular mass of 17 kDa.