Human Recombinant IFN-gamma, ACF

Interferon-gamma, animal component-free

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Interferon-gamma, animal component-free
From: 93 USD

Overview

Interferon-gamma (IFN-γ), also known as type II interferon, is produced by T and NK cells, and in smaller amounts by dendritic cells and macrophages. IFN-γ is controlled by cytokines such as IL-12 and IL-18 secreted in response to infection (Schroder et al.). IFN-γ binds to a receptor complex and initiates signal transduction via the JAK/STAT pathway; this culminates in the transcription and activation of many genes that control a diverse array of immunological functions (de Weerd and Nguyen; Krause et al.). IFN-γ stimulates the antimicrobial and anti-tumor activity of macrophages, NK cells, and neutrophils (Billiau & Matthys) by promoting the activation of microbial effector functions such as production of reactive oxygen species, NO intermediates, and complement (Schroder et al.). IFN-γ enhances MHC class I and II expression in dendritic cells and mononuclear phagocytes, as well as the production of IL-12 by dendritic cells. In B cells, IFN-γ stimulates survival and growth in both mouse and human cells, and redirects B cells from proliferation towards differentiation. IFN-γ favors the development of Th1 vs Th2 cells and stimulates monocyte differentiation and function (Schroder et al.). This product is animal component-free.
Alternative Names:
Interferon gamma, Type II interferon
Cell Type:
B Cells; Hematopoietic Stem and Progenitor Cells; Intestinal Cells; Mesenchymal Stem and Progenitor Cells; T Cells
Area of Interest:
Epithelial Cell Biology; Immunology; Stem Cell Biology
Molecular Weight:
16.9 kDa
Purity:
≥ 95%

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Data and Publications

Data

(A) The biological activity of Human Recombinant IFN-gamma, ACF was tested by measuring the survival of A549 cells infected with EMC virus. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 560 pg/mL, determined after normalization to the internal standard and calculated independently from the specific activity value. Human Recombinant IFN-gamma, ACF has a specific activity of 1.4 x 10^7 units/mg based on the calculated titer when compared to an internal standard. (B) 1 μg of Human Recombinant IFN-gamma, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.