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Data and Publications
(A) The biological activity of Human Recombinant IFN-gamma, ACF was tested by measuring the survival of A549 cells infected with EMC virus. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 560 pg/mL, determined after normalization to the internal standard and calculated independently from the specific activity value. Human Recombinant IFN-gamma, ACF has a specific activity of 1.4 x 10^7 units/mg based on the calculated titer when compared to an internal standard. (B) 1 μg of Human Recombinant IFN-gamma, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.