Human Recombinant IFN-gamma, ACF

Interferon-gamma, animal component-free

Human Recombinant IFN-gamma, ACF

Interferon-gamma, animal component-free

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Interferon-gamma, animal component-free
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Overview

Interferon-gamma (IFN-γ), also known as type II interferon, is produced by T and NK cells, and in smaller amounts by dendritic cells and macrophages. IFN-γ is controlled by cytokines such as IL-12 and IL-18 secreted in response to infection (Schroder et al.). IFN-γ binds to a receptor complex and initiates signal transduction via the JAK/STAT pathway; this culminates in the transcription and activation of many genes that control a diverse array of immunological functions (de Weerd and Nguyen; Krause et al.). IFN-γ stimulates the antimicrobial and anti-tumor activity of macrophages, NK cells, and neutrophils (Billiau & Matthys) by promoting the activation of microbial effector functions such as production of reactive oxygen species, NO intermediates, and complement (Schroder et al.). IFN-γ enhances MHC class I and II expression in dendritic cells and mononuclear phagocytes, as well as the production of IL-12 by dendritic cells. In B cells, IFN-γ stimulates survival and growth in both mouse and human cells, and redirects B cells from proliferation towards differentiation. IFN-γ favors the development of Th1 vs Th2 cells and stimulates monocyte differentiation and function (Schroder et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names

Interferon gamma, Type II interferon

Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, Intestinal Cells, Mesenchymal Stem and Progenitor Cells, T Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Immunology, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 95%

Data Figures

Figure 1. Biological Activity and Molecular Weight of Human Recombinant IFN-gamma, ACF

(A) The biological activity of Human Recombinant IFN-gamma, ACF was measured with A549 cells exposed to the encephalomyocarditis (EMC) virus in a cytopathic effect (CPE) assay. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the example above is ≤ 0.75 ng/mL. (B) 1 μg of Human Recombinant IFN-gamma, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-gamma, ACF has a predicted molecular mass of 16.9 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78141.1, 78141.3
Lot #
1000168083 and higher
Language
English
Catalog #
78141.1, 78141.3
Lot #
1000168082 and lower
Language
English
Document Type
Safety Data Sheet
Catalog #
78141.1, 78141.3
Lot #
All
Language
English