EasySep™ Mouse CD45 Positive Selection Kit
Immunomagnetic positive selection kit
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EasySep™ Mouse CD45 Positive Selection Kit -
Label for EasySep™ Mouse CD45 Positive Selection Kit
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When using the EasyEights™ EasySep™ Magnet, contact us at techsupport@stemcell.com to request an additional vial of EasySep™ Dextran RapidSpheres™ 50100.
Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Mouse CD45 Positive Selection Kit is designed to isolate CD45 cells from single-cell suspensions of splenocytes, lung, or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components:
- EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945)
- EasySep™ Mouse CD45 Positive Selection Kit Component A, 1 mL
- EasySep™ Mouse CD45 Positive Selection Kit Component B, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Innate Lymphoid Cells; Leukemia/Lymphoma Cells; Lymphocytes; Macrophages; Megakaryocytes; Monocytes; Mononuclear Cells; Myeloid Cells; NK Cells; Plasma; T Cells; T Cells, Other Subsets
Species:
Mouse
Sample Source:
Lung; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology
Related Products
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Cell dissociation reagent -
10X Collagenase/hyaluronidase in DMEM -
EasySep™ Dead Cell Removal (Annexin V) Kit
Immunomagnetic depletion cell isolation kit
Labeling Antibodies
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Anti-Mouse CD45.2 Antibody, Clone 104
Mouse monoclonal IgG2a antibody against mouse CD45.2 -
Anti-Mouse CD45.1 Antibody, Clone A20
Mouse monoclonal IgG2a antibody against mouse CD45.1
Scientific Resources
Product Documentation
Document Type
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Catalog #
Lot #
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Educational Materials
(10)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Typical EasySep™ Mouse CD45 Positive Selection Profile
(A) Starting with a naïve mouse lung single-cell suspension, the leukocyte content (CD45+) of the isolated fraction is typically 97.0% ± 1.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions in lung are 53.1% and 94.1%, respectively. (B) Starting with unlysed naïve mouse splenocytes, the leukocyte content (CD45+) of the isolated fraction is typically 97.6 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example using spleen, the purities of the start and final isolated fractions are 31.2% and 96.6%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.