Anti-Mouse CD45.2 Antibody, Clone 104

Mouse monoclonal antibody against mouse CD45.2

Anti-Mouse CD45.2 Antibody, Clone 104

Mouse monoclonal antibody against mouse CD45.2

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Mouse monoclonal antibody against mouse CD45.2
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Overview

This monoclonal antibody (clone 104) reacts with mouse alloantigen cluster of differentiation 45.2 (CD45.2), also known as Ly5.2 and leukocyte common antigen, a 180 -240 kD type I transmembrane glycoprotein. CD45.2 is an allelic form of CD45 expressed by all hematopoietic cells except mature erythrocytes and platelets in mouse strains 129, A, AKR, BALB/c, C3H/He, C57BL/6, C58, CBA, DBA/1, and DBA/2. Use this recombinant monoclonal antibody in applications requiring detection of CD45.2, such as flow cytometry (FC). This antibody clone has been verified for purity assessment of cells isolated with EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945) or EasySep™ Mouse TIL (CD45) Positive Selection Kit (Catalog #100-0350).
Clone 104 does not react with leukocytes that express the CD45.1 alloantigen. CD45 is a member of the protein tyrosine phosphatase (PTP) family and contains two catalytic domains in the intracellular (COOH) domain, which regulate cellular signaling. The extracellular domains are highly variable due to alternative splicing among exons 4, 5, and 6 as well as differential glycosylation. These CD45 isoforms correlate to specific cell types as well as specific activation and maturation states of immune cells. This monoclonal antibody has been used extensively in adoptive cell transfer and bone marrow transplantation studies in mice, allowing for the differentiation of CD45.2+ and CD45.1+ cells.
Subtype
Primary Antibodies
Target Antigen
CD45.2
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, PE
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Lymphocytes, Myeloid Cells
Species
Mouse
Application
Flow Cytometry
Area of Interest
Immunology
Clone
104
Gene ID
19264

Data Figures

Data for Biotin-Conjugated

Figure 1. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45.2 Antibody, Clone 104, Biotin followed by streptavidin (SAV) APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT) followed by SAV APC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1598
Lot #
All
Language
English
Catalog #
100-1597
Lot #
All
Language
English
Catalog #
60118BT.1, 60118BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1598
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1597
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60118BT.1, 60118BT
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications