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Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758

Rat monoclonal IgG2a, kappa isotype control antibody

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Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758

Rat monoclonal IgG2a, kappa isotype control antibody

From: 80 USD
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Rat monoclonal IgG2a, kappa isotype control antibody
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Overview

The RTK2758 antibody (IgG2a, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype
Isotype Controls
Target Antigen
IgG2a Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
RTK2758
Gene ID
Not applicable
Isotype
IgG2a, kappa

Data Figures

Data for unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Catalog #60023) followed by a mouse anti-rat IgG2a antibody, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488, Catalog #60023AD).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, APC, Catalog #60023AZ).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, Catalog #60023BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC, Catalog #60023FI).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, PE, Catalog #60023PE).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin
Catalog #
60076BT.1, 60076BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC
Catalog #
60076AZ.1, 60076AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC
Catalog #
60076FI.1, 60076FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758
Catalog #
60076.1, 60076
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488
Catalog #
60076AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE
Catalog #
60076PE, 60076PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin
Catalog #
60076BT.1, 60076BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC
Catalog #
60076AZ.1, 60076AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC
Catalog #
60076FI.1, 60076FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758
Catalog #
60076.1, 60076
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488
Catalog #
60076AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE
Catalog #
60076PE, 60076PE.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

Related Products

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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