Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071

Rat monoclonal IgG1, kappa isotype control antibody
Catalog #
60075_C
Rat monoclonal IgG1, kappa isotype control antibody
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Overview

The RTK2071 antibody (IgG1, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype
Isotype Controls
Target Antigen
IgG1 Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
Other
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
RTK2071
Gene ID
Not applicable
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071
Catalog #
60075, 60075.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488
Catalog #
60075AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC
Catalog #
60075AZ, 60075AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin
Catalog #
60075BT, 60075BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC
Catalog #
60075FI, 60075FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE
Catalog #
60075PE, 60075PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071
Catalog #
60075, 60075.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488
Catalog #
60075AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC
Catalog #
60075AZ, 60075AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin
Catalog #
60075BT, 60075BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC
Catalog #
60075FI, 60075FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE
Catalog #
60075PE, 60075PE.1
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5 (Catalog #60009), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC (Catalog #60009AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin (Catalog #60009BT) followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE (Catalog #60009PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

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