Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071
Rat monoclonal IgG1, kappa isotype control antibody
Request Pricing
Thank you for your interest in this product. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
Overview
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Data Figures
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5 (Catalog #60009), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.
Figure 3. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC (Catalog #60009AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 4. Data for Biotin-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin (Catalog #60009BT) followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.
Figure 5. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE (Catalog #60009PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Related Products
- Mouse IgG1, kappa Isotype Control Antibody, C...
Mouse monoclonal IgG1, kappa isotype control antibody
- Rat IgM, kappa Isotype Control Antibody, Clon...
Rat monoclonal IgM, kappa isotype control antibody
- Rat IgG2a, kappa Isotype Control Antibody, Cl...
Rat monoclonal IgG2a, kappa isotype control antibody
- Rat IgG2b, kappa Isotype Control Antibody, Cl...
Rat monoclonal IgG2b, kappa isotype control antibody
Item added to your cart
Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.