EasySep™ Mouse TIL (CD45) Positive Selection Kit

Immunomagnetic positive selection kit

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EasySep™ Mouse TIL (CD45) Positive Selection Kit

Immunomagnetic positive selection of mouse CD45+ TILs from single-cell suspensions of solid tumors

1 x 109 cells
Catalog #100-0350
655 USD

Required Products

Overview

The EasySep™ Mouse TIL (CD45) Positive Selection Kit is designed to isolate highly purified CD45+ tumor infiltrating leukocytes (TILs) by immunomagnetic positive selection. This kit has been optimized for use on single-cell suspensions of solid mouse tumors with a low CD45+ start cell frequency, including tumors induced by implantation of 4T1, B16-F10, and CT26.WT cell lines into syngeneic mice. Due to the heterogeneity of mouse tumors, this kit may require optimization.

This kit targets CD45+ cells for positive selection with antibodies recognizing the CD45 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, and cell-based experiments.
Advantages:
⦁ Fast, easy to use, and column-free
⦁ Optimized for tumors and tissue samples with a low CD45 start frequency
⦁ Flexible protocols for higher purity or recovery
Components:
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component A, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component B, 0.25 mL
  • EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component C, 1.5 mL
  • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
Leukocytes
Species:
Mouse
Sample Source:
Other
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Cancer Research; Immunology

Scientific Resources

Educational Materials

(10)

Data and Publications

Data

Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension. Using the EasySep™ Mouse TIL (CD45) Positive Selection Kit, the CD45+ TIL purities of the start and final isolated fractions are 14.8% and 95.2%, respectively for B16-F10 tumor single-cell suspension, 37.6% and 93.0%, respectively for 4T1 tumor single-cell suspension, and 32.3% and 84.6%, respectively for CT26.WT tumor single-cell suspension.

Figure 1. EasySep™ Mouse TIL (CD45) Positive Selection Kit

Tumors were induced by B16-F10, 4T1, or CT26.WT cell lines and dissociated into single-cell suspensions. CD45+ TILs were isolated from single-cell suspensions at various start concentrations using the purple EasySep™ Magnet. (A) Starting with a B16-F10 tumor single-cell suspension at 1 x 10^8 cells/mL, the purities of the start and final isolated fractions are 14.8% and 95.2%, respectively. (B) Starting with a 4T1 tumor single-cell suspension at 4 x 10^7 cells/mL, the purities of the start and final isolated fractions are 37.6% and 93.0%, respectively. (C) Starting with a CT26.WT tumor single-cell suspension at 2.5 x 10^7 cells/mL, the purities of the start and final isolated fractions are 32.3% and 84.6%, respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.