This kit targets CD45+ cells for positive selection with antibodies recognizing the CD45 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, and cell-based experiments.
⦁ Optimized for tumors and tissue samples with a low CD45 start frequency
⦁ Flexible protocols for higher purity or recovery
- EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component A, 0.25 mL
- EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component B, 0.25 mL
- EasySep™ Mouse TIL (CD45) Positive Selection Cocktail Component C, 1.5 mL
- EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. EasySep™ Mouse TIL (CD45) Positive Selection Kit
Tumors were induced by B16-F10, 4T1, or CT26.WT cell lines and dissociated into single-cell suspensions. CD45+ TILs were isolated from single-cell suspensions at various start concentrations using the purple EasySep™ Magnet. (A) Starting with a B16-F10 tumor single-cell suspension at 1 x 10^8 cells/mL, the purities of the start and final isolated fractions are 14.8% and 95.2%, respectively. (B) Starting with a 4T1 tumor single-cell suspension at 4 x 10^7 cells/mL, the purities of the start and final isolated fractions are 37.6% and 93.0%, respectively. (C) Starting with a CT26.WT tumor single-cell suspension at 2.5 x 10^7 cells/mL, the purities of the start and final isolated fractions are 32.3% and 84.6%, respectively.