EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection kit

More Views

EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection of CD45+ leukocytes from mouse lung, splenocytes, or other tissues

2 x 109 cells
Catalog #18945
704 USD

When using the EasyEights™ EasySep™ Magnet, contact us at techsupport@stemcell.com to request an additional vial of EasySep™ Dextran RapidSpheres™ 50100.

Required Products

Overview

The EasySep™ Mouse CD45 Positive Selection Kit is designed to isolate CD45 cells from single-cell suspensions of splenocytes, lung, or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components:
  • EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945)
    • EasySep™ Mouse CD45 Positive Selection Kit Component A, 1 mL
    • EasySep™ Mouse CD45 Positive Selection Kit Component B, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Innate Lymphoid Cells; Leukemia/Lymphoma Cells; Lymphocytes; Macrophages; Megakaryocytes; Monocytes; Mononuclear Cells; Myeloid Cells; NK Cells; Plasma; T Cells; T Cells, Other Subsets
Species:
Mouse
Sample Source:
Lung; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(10)

Data and Publications

Data

FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit

Figure 1. Typical EasySep™ Mouse CD45 Positive Selection Profile

(A) Starting with a naïve mouse lung single-cell suspension, the leukocyte content (CD45+) of the isolated fraction is typically 97.0% ± 1.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions in lung are 53.1% and 94.1%, respectively. (B) Starting with unlysed naïve mouse splenocytes, the leukocyte content (CD45+) of the isolated fraction is typically 97.6 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example using spleen, the purities of the start and final isolated fractions are 31.2% and 96.6%, respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.