NeuroCult™

For Primary Neuronal and Neural Stem Cell Culture

NeuroCult™ is a line of culture media and reagents formulated to provide optimal culture conditions for primary neurons, CNS-derived neural stem and progenitor cells, and brain tumor stem cells. NeuroCult™ allows researchers to standardize their culture system and minimize variability, leading to increased reproducibility among experiments.

Why Use NeuroCult™?

  • Media, supplements and accessory reagents are available for primary neurons and neural stem/progenitor cells from human, mouse and rat tissues.
  • Isolate, expand, quantify and differentiate mouse and rat neural stem and progenitor cells from normal and tumor tissues.
  • Culture and expand human neural stem cells and brain tumor stem cells in a serum-free and/or xeno-free system.
  • Rigorous raw material screening and quality control ensure minimal lot-to-lot variability.

For the generation and culture of neural cells derived from human pluripotent stem cells (hPSCs; including embryonic stem (ES) cell and induced pluripotent stem (iPS) cells), see STEMdiff™ and BrainPhys™, which are part of the hPSC-derived neural cell research workflow.



NeuroCult™ for CNS-Derived Mouse and Rat Neural Stem and Progenitor Cells

NeuroCult™ Proliferation Kits

Species:

Mouse or Rat

Formulation:

Defined and serum-free

Applications:

Features/Advantages:

Efficient expansion of neural stem and progenitor cells without loss of self-renewal, proliferation or differentiation potential

Notes:

Must be supplemented as follows:
  • Embryonic mouse: rh EGF
  • Adult mouse: rh EGF, rh FGF and heparin
  • Embryonic rat: rh EGF, rh FGF and heparin

NeuroCult™ Differentiation Kits

Species:

Mouse or Rat

Formulation:

Serum-containing

Applications:

Features/Advantages:

Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes

NeuroCult™ Dissociation Kits

Species:

Mouse (Chemical Dissociation); Mouse and Rat (Enzymatic Dissociation)

Formulation:

Defined and serum-free

Applications:

Features/Advantages:

Fast, reproducible and yields high total cell numbers and good cell viability

NeuroCult™ Quantification Kits

Species:

Mouse or Rat

Formulation:

Defined and serum-free

Applications:

Features/Advantages:

Enables discrimination between neural stem and progenitor cells

Notes:

Must be supplemented as follows:
  • Embryonic mouse: rh EGF
  • Adult mouse: rh EGF, rh FGF and heparin
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NeuroCult™ SM1 for Primary and hPSC-Derived Neurons

NeuroCult™ SM1 Neuronal Supplement

NeuroCult™ SM1 Neuronal Supplement

Species:

Mouse and Rat Primary Neurons, Human ES/iPS Cell-Derived Neurons

Formulation:

Defined and serum-free supplement based on Brewer’s B271

Applications:

  • Culture of primary mouse and rat neurons
  • Differentiation and maturation of human ES/iPS cell-derived neurons*
  • Versatile serum-replacement supplement for various applications

Features/Advantages:

  • Formulated to support improved cell survival in long-term primary neuronal culture
  • Cultures feature increased neurite outgrowth and branching in short- and long-term cultures
  • Product undergoes rigorous performance testing

NeuroCult™ SM1 Neuronal Culture Kit

Species:

Mouse and Rat

Formulation:

Defined and serum-free basal medium and supplement based on Brewer’s Neurobasal and B271

Applications:

Traditional culture of primary mouse and rat neurons

Features/Advantages:

  • Complete kit formulated to support improved cell survival in long-term primary neuronal cultures
  • Cultures feature increased neurite outgrowth and branching in short- and long-term cultures
  • Product undergoes rigorous performance testing with primary neuronal cultures

Notes:

Kit includes NeuroCult™ Neuronal Basal Medium and NeuroCult™ SM1 Neuronal Supplement

BrainPhys™ Neuronal Medium and SM1 Kit

BrainPhys™ Neuronal Medium and SM1 Kit

Species:

Mouse and Rat

Formulation:

Defined and serum-free basal medium and supplement

BrainPhys™ Neuronal Medium is based on the formulation invented by Cedric Bardy and Fred H. Gage2

Applications:

  • Neurophysiological culture of primary mouse and rat neurons
  • Differentiation and maturation of functionally active human ES/iPS cell-derived neurons*
  • Microelectrode array-based recording of neuronal activity

Features/Advantages:

  • Complete kit formulated to support improved neuronal function in long-term primary and human ES/iPS cell-derived neuronal cultures
  • Cultures experience brain-like physiological conditions and have a higher proportion of synaptically active neurons
  • Perform functional assays without replacing media
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NeuroCult™ for Human Neural Stem Cells and Brain Tumor Stem Cells

NeuroCult™ NS-A Proliferation Kit

Species:

Human

Formulation:

Defined and serum-free

Applications:

Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers

Features/Advantages:

Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential

Notes:

Must be supplemented with:
  • rh EGF
  • rh bFGF
  • Heparin

NeuroCult™ Xeno-Free Proliferation Medium

Species:

Human

Formulation:

Defined, xeno-free and serum-free

Applications:

Culture and expansion of human neural stem and progenitor cells or brain tumor stem cells as neurospheres or adherent monolayers

Features/Advantages:

Efficient expansion in culture without loss of self-renewal, proliferation or differentiation potential

Notes:

Must be supplemented with:
  • rh EGF
  • rh bFGF
  • Heparin

NeuroCult™ NS-A Differentiation Kit

Species:

Human

Formulation:

Serum-containing

Applications:

Differentiation of human neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes

Features/Advantages:

Efficient and reproducible differentiation to neurons, astrocytes and oligodendrocytes
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