Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-49 (tissue non-specific alkaline phosphatase)
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-49 (tissue non-specific alkaline phosphatase), unconjugated

100 µg
Catalog # 60066
454 USD

Overview

The TRA-2-49/6E antibody reacts with the tissue non-specific isoform of alkaline phosphatase (AP), which is expressed at high levels in undifferentiated pluripotent stem cells such as human and mouse embryonic stem (ES) cells, as well as induced pluripotent stem (iPS) and embryonal carcinoma (EC) cells. This isozyme of AP is the same as that expressed by liver, bone and kidney cells. Levels of AP decrease upon differentiation and, thus, the TRA-2-49/6E antibody can be used to monitor the differentiation status of pluripotent stem cells. The TRA-2-49/6E antibody does not cross-react with other isozymes of AP, which differ through post-translational modification, and reportedly does not recognize AP from cow, dog, goat, Guinea pig, hamster, mouse, rat or sheep, or Old World Monkeys such as the rhesus monkey, baboon and African green monkey.
Subtype
Primary Antibodies
Target Antigen
TRA-2-49
Alternative Names
Liver/bone/kidney alkaline phosphatase, tissue non-specific alkaline phosphatase
Reactive Species
Non-Human Primate, Cat, Human, Pig, Rabbit
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Pluripotent Stem Cells
Species
Human, Non-Human Primate, Other
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Stem Cell Biology
Clone
TRA-2-49/6E
Gene ID
249
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E
Catalog #
60066
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E
Catalog #
60066
Lot #
All
Language
English

Educational Materials (3)

Brochure
Reliable Antibodies for Your Research
Brochure
Products for Human Pluripotent Stem Cells
Brochure
Standardized Solutions for Mouse Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiHuman TRA-2-49 Antibody, Clone TRA-2-49/6E, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Labeling of human ES cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody, followed by goat anti-mouse IgG, FITC. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (negative control, lane 2) with Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E.

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