Anti-Human SSEA-5 Antibody, Clone 8e11
Mouse monoclonal IgG1 antibody against human SSEA-5
Overview
Data Figures
Figure 1. Data for FITC-Conjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.
(C) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human SSEA-5 Antibody, Clone 8e11.
Figure 2. Data for PE-Conjugated
(A) Flow cytometry analysis of human embryonic stem (ES) cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Labeling of human ES cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
(B) Flow cytometry analysis of human induced pluripotent stem (iPS) cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Labeling of human iPS cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
(C) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Inset shows labeling of human ES cells with a mouse IgG1, kappa PE isotype control antibody.
(D) Human iPS cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, PE. Inset shows labeling of human iPS cells with a mouse IgG1, kappa PE isotype control antibody.
Figure 3. Data for Unconjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human SSEA-5 Antibody, Clone 8e11, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC. (C) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human SSEA-5 Antibody, Clone 8e11.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Related Products
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
