Human Recombinant IL-2 (E. coli-expressed)

Interleukin 2
Catalog #
78220_C
Interleukin 2
From: 80 USD

Overview

Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor (Gaffen & Liu). It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival (Ma et al.). The majority of IL-2 is secreted by activated CD4+ and CD8+ T cells, although B cells and dendritic cells were found to produce IL-2 in small amounts. IL-2 downregulates immune responses to prevent autoimmunity during thymic development, influences the development of CD4+CD25+ T regulatory cells, and affects development of follicular helper T cells. IL-2 also controls inflammation by inhibiting Th17 differentiation (Banchereau et al.). High IL-2 levels in serum are associated with progression of scleroderma, rheumatoid arthritis, and gastric and non-small cell lung cancer, though no known disease can be directly attributed to the lack or excess of IL-2 (Gaffen & Liu). Human Recombinant IL-2 has a serine substitute for cysteine at position 126.
Subtype
Cytokines
Alternative Names
Aldesleukin, Interleukin-2, T cell growth factor, TCGF
Cell Type
Hematopoietic Stem and Progenitor Cells, NK Cells, T Cells, T Cells, CD4+, T Cells, CD8+
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
15.5 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-2 (E. coli-expressed)
Catalog #
78220, 78220.1, 78220.2, 78220.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-2 (E. coli-expressed)
Catalog #
78220, 78220.1, 78220.2, 78220.3
Lot #
All
Language
English

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-2 was tested by its ability to promote the proliferation of CTLL-2 cells. Cell proliferation was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.99 ng/mL. (B) 1 μg of Human Recombinant IL-2 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-2 has a predicted molecular mass of 15.5 kDa.

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