Human Recombinant IL-12

Interleukin 12
Catalog #
78027_C
Interleukin 12
From: 144 USD

Overview

Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann & Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation, and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).
Subtype
Cytokines
Alternative Names
CTL maturation factor, Cytotoxic lymphocyte maturation factor, IL-12p70, Interleukin-12, Natural killer cell stimulatory factor, NKSF
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, Lymphocytes, NK Cells, T Cells
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
p35 Subunit: 22.5 kDa; p40 Subunit: 34.7 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-12
Catalog #
78027, 78027.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-12
Catalog #
78027, 78027.1
Lot #
All
Language
English

Educational Materials(3)

Brochure
Tools For Your Immunology Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Human Immune Cytokines

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-12 was tested by its ability to produce IFN-gamma in NK cells co-stimulated with IL18. The EC50 is defined as the effective concentration of the growth factor at which IFN-gamma production is at 50% of maximum. The EC50 in the above example is less than 2 ng/mL.
(B) 2 μg of Human Recombinant IL-12 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-12 has a predicted molecular mass of 22.5 - 34.7 kDa.

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