Human Recombinant IFN-gamma

Interferon-gamma

Human Recombinant IFN-gamma

Interferon-gamma

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Interferon-gamma
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Overview

Interferon-gamma (IFN-γ), also known as type II interferon, is produced by T and NK cells, and in smaller amounts by dendritic cells and macrophages. IFN-γ is controlled by cytokines such as IL-12 and IL-18 secreted in response to infection (Schroder et al.). IFN-γ binds to a receptor complex and initiates signal transduction via the JAK/STAT pathway; this culminates in the transcription and activation of many genes that control a diverse array of immunological functions (de Weerd & Nguyen; Krause et al.). IFN-γ stimulates the antimicrobial and anti-tumor activity of macrophages, NK cells, and neutrophils (Billiau & Matthys) by promoting the activation of microbial effector functions such as production of reactive oxygen species, nitric oxide, and complement (Schroder et al.). IFN-γ enhances MHC class I and II expression in dendritic cells and mononuclear phagocytes, as well as the production of IL-12 by dendritic cells. In B cells, IFN-γ stimulates survival and growth in both mouse and human cells, and redirects B cells from proliferation towards differentiation. IFN-γ favors the development of Th1 vs Th2 cells and stimulates monocyte differentiation and function (Schroder et al.).
Subtype
Cytokines
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, Intestinal Cells, Mesenchymal Stem and Progenitor Cells, T Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Immunology, Stem Cell Biology
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant IFN-gamma was tested by its ability to promote the cytotoxicity of HT-29 (HTB-38) cells. Cytotoxicity was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell death is at 50% of maximum. The EC50 for this effect is 0.035 ng/mL.
(B) 2 μg of Human Recombinant IFN-gamma was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN-gamma has a predicted molecular mass of 17 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78020.1, 78020.2, 78020
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78020.1, 78020.2, 78020
Lot #
All
Language
English

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