Make more informed purchasing decisions with our new product availability and delivery estimate feature, now available on all product pages, in your cart, and during checkout.
Sign In
New to STEMCELL?
Register for an account to quickly and easily purchase products online and for one-click access to all educational content.
Thank you for your interest in this product.
Please provide us with your contact information and your local representative
will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
Receptor activator of nuclear factor kappa-B ligand (RANKL) is a member of the tumor necrosis factor (TNF) superfamily (Anderson et al.). Cytokines in the TNF superfamily are involved in a variety of long-term cellular activities such as differentiation, proliferation, and cell death (MacEwan). RANKL is a type II homotrimeric transmembrane protein expressed in both a membrane-bound and secreted form (Ikeda et al.). RANKL binds to the receptor activator of nuclear factor kappa-B (RANK). Upon binding to its receptor, RANKL activates the AKT signaling pathway (Moon et al.). Osteoprotegerin (OPG) may also bind RANKL, and this binding competes with RANKL-RANK binding (Lacey et al.). RANKL is involved in osteoclastogenesis (Lacey et al.; Yasuda et al.) and T cell activation (Wong et al.).
Subtype
Cytokines
Alternative Names
CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoclast differentiation factor, soluble Receptor activator of NF-κB ligand, sOdf, TNF-related activation-induced cytokine, TNFSF11, TNF superfamily member 11, TNLG6B, Tumor necrosis factor superfamily member 11, TRA
Cell Type
Airway Cells, Cancer Cells and Cell Lines, Dendritic Cells, Intestinal Cells, Mammary Cells, Osteoblasts, Other, Pancreatic Cells, Prostate Cells, T Cells
(A) The biological activity of Human Recombinant RANKL was tested by its ability to induce production of embryonic alkaline phosphatase in RAW-Blue™ macrophage reporter cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the above example is 38.8 ng/mL.
(B) Human Recombinant RANKL was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant RANKL has a predicted molecular mass of 19.7 kDa.
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
