Human Recombinant PDGF-AB

Platelet-derived growth factor AB

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Platelet-derived growth factor AB
From: 202 USD

Overview

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark & Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin- like fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). It has been shown that PDGF-AB together with 5-Azacytidine (Catalog #72012), induces the conversion of mature bone and fat cells into tissue-regenerative multipotent stem cells (Chandrakanthan et al.).
Alternative Names:
GDGF, Glioma-derived growth factor, ODGF, Osteosarcoma-derived growth factor, Platelet-derived growth factor-AB
Cell Type:
Mesenchymal Cells, PSC-Derived; Neural Cells, PSC-Derived; Neural Stem and Progenitor Cells; Neurons; Other; Osteoblasts
Species:
Human
Area of Interest:
Neuroscience; Stem Cell Biology
Molecular Weight:
14.4 kDa alpha monomer, 12.4 kDa beta monomer; 26.8 kDa dimer
Purity:
≥ 95%

Scientific Resources

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Data and Publications

Data

(A) The biological activity of Human Recombinant PDGF-AB was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured after 46 hours using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 1.8 ng/mL. (B) 1 μg of Human Recombinant PDGF-AB was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant PDGF-AB is a heterodimer of one 14.4 kDa alpha-chain and 12.4 kDa beta-chain with a predicted total molecular mass of 26.8 kDa.

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