Anti-Human CD90 Antibody, Clone 5E10

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD90 (Thy-1)

Anti-Human CD90 Antibody, Clone 5E10

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD90 (Thy-1)

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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD90 (Thy-1)
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Overview

The 5E10 antibody reacts with CD90 (Thy-1), a GPI-linked membrane glycoprotein that is N-glycosylated at two sites, giving rise to 25 - 37 kDa molecules. CD90 has roles in signal transduction, cell adhesion and migration, neurite outgrowth, T cell activation, tumor suppression, and inhibition of the proliferation and differentiation of hematopoietic stem cells. It is a known ligand of β2 and β3 integrins and upregulates synthesis of fibronectin, osteonectin and thrombospondin. CD90 is broadly expressed, being found on human thymocytes, neurons, some glial cells, fibroblasts, activated endothelial cells, some leukemia cell lines and a distinct subset (<1%) of CD3+CD4+ T cells in human peripheral blood. CD90 is also expressed by small subsets of CD34+ cells in fetal liver, umbilical cord blood, bone marrow and mobilized peripheral blood cells. CD90 is considered an important marker for hematopoietic stem and progenitor cells and, in combination with other markers such as CD34, is useful to identify and isolate these cells by FACS.

This antibody clone has been verified for labeling human mesenchymal cells grown in MesenCult™ Proliferation Kit (Human; Catalog #05411) and MesenCult™-XF Medium (Catalog #05420).
Subtype
Primary Antibodies
Target Antigen
CD90 (Thy-1)
Alternative Names
CDw90, Thy-1, Thy1
Reactive Species
Baboon, Cynomolgus, Dog, Human, Macaque, Pig, Rhesus
Conjugation
APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
Hematopoietic Stem and Progenitor Cells, Mammary Cells, Mesenchymal Stem and Progenitor Cells
Species
Human, Non-Human Primate, Other
Application
CyTOF, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Clone
5E10
Gene ID
7070
Isotype
IgG1, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD90 Antibody, Clone 5E10, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD90 Antibody, Clone 5E10, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human HEL cells labeled with Anti-Human CD90 Antibody, Clone 5E10, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of human HEL cells labeled with Anti-Human CD90 Antibody, Clone 5E10, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of human HEL cells labeled with Anti-Human CD90 Antibody, Clone 5E10, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human erythroleukemina (HEL) cells labeled with Anti-Human CD90 Antibody, Clone 5E10, PerCP-Cy5.5 (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PerCP-Cy5.5 (Catalog # 60070PS) (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60045BT
Lot #
All
Language
English
Catalog #
60045FI, 60045FI.1
Lot #
All
Language
English
Catalog #
60045PE, 60045PE.1
Lot #
All
Language
English
Catalog #
60045
Lot #
All
Language
English
Catalog #
60045PS, 60045PS.1
Lot #
All
Language
English
Catalog #
60045AZ.1, 60045AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60045BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60045FI, 60045FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60045PE, 60045PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60045
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60045PS, 60045PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60045AZ.1, 60045AZ
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Publications (1)

Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Wu X et al. Cell 2018 JAN

Abstract

Stem cells are highly resistant to viral infection compared to their differentiated progeny; however, the mechanism is mysterious. Here, we analyzed gene expression in mammalian stem cells and cells at various stages of differentiation. We find that, conserved across species, stem cells express a subset of genes previously classified as interferon (IFN) stimulated genes (ISGs) but that expression is intrinsic, as stem cells are refractory to interferon. This intrinsic ISG expression varies in a cell-type-specific manner, and many ISGs decrease upon differentiation, at which time cells become IFN responsive, allowing induction of a broad spectrum of ISGs by IFN signaling. Importantly, we show that intrinsically expressed ISGs protect stem cells against viral infection. We demonstrate the in vivo importance of intrinsic ISG expression for protecting stem cells and their differentiation potential during viral infection. These findings have intriguing implications for understanding stem cell biology and the evolution of pathogen resistance.