Anti-Human CD51 Antibody, Clone NKI-M9

Mouse monoclonal IgG2a antibody against human CD51 (integrin αV)

Anti-Human CD51 Antibody, Clone NKI-M9

Mouse monoclonal IgG2a antibody against human CD51 (integrin αV)

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Mouse monoclonal IgG2a antibody against human CD51 (integrin αV)
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Overview

The NKI-M9 antibody reacts with CD51 (vitronectin receptor α chain, or integrin αV), a type I transmembrane glycoprotein containing two subunits (125 kDa and 24 kDa) which are generated by post-translational cleavage and are linked by a disulfide bond. CD51 associates non-covalently with integrin β1 (CD29), β3 (CD61), β5, β6, or β8 to form heterodimeric cell adhesion receptors for extracellular matrix components such as fibrinogen, collagen, fibronectin, laminin, osteopontin, thrombospondin, vitronectin, and von Willebrand factor. For example, association of CD51 and CD61 forms the integrin αv/β3 receptor primarily involved in binding vitronectin. In addition to mediating adhesion and cytoskeletal organization, CD51-containing integrins have roles in signal transduction and thereby modulate processes such as cell proliferation, differentiation, and migration. Both ligand binding and ligand-induced receptor clustering are necessary for initiating integrin-mediated responses. CD51 is expressed broadly on many types of cells, including endothelial cells, fibroblasts, monocytes, macrophages, platelets (at relatively low levels), and osteoclasts. It is also found on hepatoma, melanoma, and neuroblastoma cells.
Subtype
Primary Antibodies
Target Antigen
CD51 (Integrin αV)
Alternative Names
αV integrin, integrin αV, vitronectin receptor α chain
Reactive Species
Human
Conjugation
Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human
Application
ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation
Area of Interest
Stem Cell Biology
Clone
NKI-M9
Gene ID
3685
Isotype
IgG2a, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of HT1080 human fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC­173 (Catalog #60071), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

Flow cytometry analysis of human HT-1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, FITC (filled historgram) or a mouse IgG2a, kappa FITC isotype control antibody (open histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, PE (filled histogram) or a mouse IgG2a, kappa PE isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human HT1080 fibrosarcoma cells labeled with Anti-Human CD51 Antibody, Clone NKI-M9, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60043FI, 60043FI.1
Lot #
All
Language
English
Catalog #
60043BT
Lot #
All
Language
English
Catalog #
60043
Lot #
All
Language
English
Catalog #
60043PE, 60043PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043FI, 60043FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60043PE, 60043PE.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)