Directed Differentiation and Disease Modeling

Human pluripotent stem cell (hPSC)-based models hold tremendous potential for studying human development and disease. Directed differentiation and disease modeling are two key methods for using hPSCs in drug discovery, cell therapy validation, and disease research.

• Directed differentiation of hPSCs refers to the in vitro differentiation of these cells toward a specific cell type through defined cell culture conditions. Directed differentiation is achieved by the addition of specific growth factors or small molecules.
• Disease modeling is an approach to study diseases using cells that display relevant pathological features. Disease modeling using hPSCs can be achieved by reproduction of a disorder-associated mutation with gene editing, isolation of embryonic stem cells (ESCs) from affected blastocysts; or generation of induced pluripotent stem cells (iPSCs) from patients’ somatic cells.

Explore the resources below to support your disease modeling studies using hPSCs.

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Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differentiation

Recorded during the ISSCR 2017 Innovation Showcase in Boston, this tutorial highlights human pluripotent stem cell (hPSC) gene-editing and cardiac differentiation workflows using the CloneR™ supplement and the STEMdiff™ Cardiomyocyte System. This talk is presented by STEMCELL Technologies’ Dr. Adam Hirst, scientist for pluripotent stem cell biology, and Dr. Vincenzo Macri, senior scientist for cardiomyocyte stem cell biology.

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