Quality Control

Know how to characterize your pluripotent stem cells, for confidence in your cultures.

Impact of Genomic Integrity

hPSCs frequently acquire abnormalities in a non-random and sporadic manner. Some abnormalities are also observed in human cancers, raising concerns about progression into clinical trials. Understanding and detecting these changes is key to ensuring safety and efficacy of future therapies.
  • When hPSCs Display Hallmarks of Cancer: Hear Dr. Peter Andrews and Dr. Ivana Barbaric from the University of Sheffield discuss the causes and consequences of genetic changes in hPSCs, including acquisition of genetic changes that render them less susceptible to differentiation and/or death. View Now >

Assessing Pluripotency

The teratoma assay is the most widely documented technique for evaluating pluripotency. However, it can be lengthy, cost prohibitive, and follow variable methodologies and analyses. Alternative approaches such as trilineage differentiation provide researchers with more ease-of-use and specificity.
  • Validation Through Trilineage Differentiation: Advances in protocols for directed differentiation towards the primary germ layers now allow researchers to assess stem cell pluripotency in vitro without a teratoma assay. View Now >

Controlling the Culture System

The maintenance and propagation of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells requires the use of optimized media formulations, in combination with careful handling techniques, to maintain high-quality cultures at each passage.
  • Ensuring Optimal pH of hPSC Culture Medium: See this tech tip to learn about the potential impacts of acidification on hPSC culture, and what to consider to maintain optimal pH. View Now >

Mastering Pluripotent Stem Cell Quality Characterization

In high quality cultures, certain cell surface and intracellular markers should be homogeneously expressed in nearly all cells.

Explore Gene & Marker Expression >

Due to transient epigenetic memory, using only iPS cells that are fully reprogrammed and characterized is important to avoid aberrations.

Explore Epigenetic Landscape >

Genome Editing of hPSCs

New tools, such as the CRISPR-Cas9 system, have improved the ease and efficiency of engineering hPSCs. However, off-target effects are currently a major concern in genome editing. To increase specificity, modified versions of wild-type Cas9 are being developed.
  • In this webinar, Dr. Chad Cowan from Harvard University discusses the design and use of TALENs or CRISPR-Cas9 systems with hPSCs. He also covers the applications of modified hPSCs, including disease modeling and gene therapy. View Now >