DisCoZyme 2, ACF

Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity

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DisCoZyme 2, ACF

Collagenase/dispase blend with high collagenase and high caseinase activity

50 mg
Catalog #07450
379 USD

Overview

DisCoZyme 2, Animal Component-Free (ACF) is a blend of collagenase and dispase, both of which are obtained from cultures free of animal-derived materials. DisCoZyme 2, ACF contains a minimum of 250 collagenase units/mg and 2000 caseinase units/mg of dry weight. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases, including collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Dispase is a neutral, amino-endoprotease that cleaves the N-terminal peptide bond of non-polar amino acid residues. Dispase has mild proteolytic activity, which makes it especially useful for the isolation and passaging of primary cells. Its proteolytic activity also allows it to maintain membrane integrity. DisCoZyme 2, ACF is designed to contain high levels of collagenase and caseinase activity and is intended for use in applications where prevention of the introduction of potential animal-derived pathogens is necessary. DisCoZyme 2, ACF has been used for the digestion of tissues such as lung (Fujino et al.; Driscoll et al.) and neural (Panchision et al.).
Subtype:
Enzymatic
Alternative Names:
Collagenase mix; Discozyme; Dispase mix; Collagenase/dispase mix; Neutral protease; Proteinase
Cell Type:
Airway Cells; Neural Stem and Progenitor Cells
Species:
Human; Mouse; Rat; Non-Human Primate; Other
Area of Interest:
Neuroscience; Respiratory Research
Molecular Weight:
32 - 130 kDa

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.