DisCoZyme 1, ACF

Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity

DisCoZyme 1, ACF

Animal component-free collagenase/dispase blend with high collagenase and high caseinase activity

DisCoZyme 1, ACF
50 mg
238 USD
Catalog # 07449

Collagenase/dispase blend with high collagenase and high caseinase activity

Overview

DisCoZyme 1, Animal Component-Free (ACF) is a blend of collagenase and dispase, both of which are obtained from cultures free of animal-derived materials. DisCoZyme 1, ACF contains a minimum of 250 collagenase units/mg and 1000 caseinase units/mg of dry weight. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases, including collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Dispase is a neutral, amino-endoprotease that cleaves the N-terminal peptide bond of non-polar amino acid residues. Dispase has mild proteolytic activity, which makes it especially useful for the isolation and passaging of primary cells. Its proteolytic activity also allows it to maintain membrane integrity. DisCoZyme 1, ACF is designed to contain high levels of collagenase and caseinase activity and is intended for use in applications where prevention of the introduction of potential animal-derived pathogens is necessary. DisCoZyme 1, ACF has been used for the digestion of tissues such as lung (Fujino et al.; Driscoll et al.) and neural (Panchision et al.).
Subtype
Enzymatic
Alternative Names
Collagenase mix; Discozyme; Dispase mix; Collagenase/dispase mix; Neutral protease; Proteinase
Cell Type
Airway Cells, Neural Stem and Progenitor Cells
Species
Human, Mouse, Rat, Non-Human Primate, Other
Area of Interest
Neuroscience, Respiratory Research
Molecular Weight
32 - 130 kDa

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
DisCoZyme 1, ACF
Catalog #
07449
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DisCoZyme 1, ACF
Catalog #
07449
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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