DisCoZyme 1, Animal Component-Free (ACF) is a blend of collagenase and dispase, both of which are obtained from cultures free of animal-derived materials. DisCoZyme 1, ACF contains a minimum of 250 collagenase units/mg and 1000 caseinase units/mg of dry weight. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases, including collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Dispase is a neutral, amino-endoprotease that cleaves the N-terminal peptide bond of non-polar amino acid residues. Dispase has mild proteolytic activity, which makes it especially useful for the isolation and passaging of primary cells. Its proteolytic activity also allows it to maintain membrane integrity. DisCoZyme 1, ACF is designed to contain high levels of collagenase and caseinase activity and is intended for use in applications where prevention of the introduction of potential animal-derived pathogens is necessary. DisCoZyme 1, ACF has been used for the digestion of tissues such as lung (Fujino et al.; Driscoll et al.) and neural (Panchision et al.).