ArciTect™ High-Fidelity DNA Polymerase Kit

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

ArciTect™ High-Fidelity DNA Polymerase Kit

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification

From: 565 USD
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Polymerase, buffers, and dNTPs for high-fidelity PCR amplification
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What's Included

  • ArciTect™ High-Fidelity DNA Polymerase, 250 µL
  • ArciTect™ High-Fidelity Buffer, 6 x 1.5 mL
  • ArciTect™ High GC Content Buffer, 3 x 1.5 mL
  • dNTP Mix, 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

ArciTect™ High-Fidelity DNA Polymerase Kit is optimized for robust, high-fidelity DNA amplification (> 50X higher than Taq). It may be used in applications requiring ultra-low error rates, such as detection of genome editing with ArciTect™ T7 Endonuclease I (refer to the Technical Bulletin: Evaluation of Genome Editing), as well as sequencing, cloning/subcloning, synthetic biology, and SNP analysis. ArciTect™ High-Fidelity DNA Polymerase is a fusion protein with a double-stranded DNA-binding domain and a Pyrococcus-like proofreading polymerase domain.

ArciTect™ High-Fidelity DNA Polymerase Kit includes ArciTect™ High-Fidelity DNA Polymerase, dNTP Mix (10 mM; containing dATP, dCTP, dGTP, and dTTP sodium salts), and two buffers: ArciTect™ High-Fidelity Buffer (for standard high-fidelity reactions) and ArciTect™ High GC Content Buffer (for difficult-to-amplify templates that are rich in G and C bases). Reactions using ArciTect™ High GC Content Buffer have increased sensitivity with a slightly higher error rate. All components are animal component-free.

ArciTect™ High-Fidelity DNA Polymerase exhibits a 50- to 60-fold lower error rate than standard Taq polymerase using the lacl mutagenesis assay. It has an extension rate of 67 nucleotides per second and can successfully amplify long targets (up to 5 kb human genomic DNA and 8 kb of lambda DNA). This enzyme generates blunt-end products.
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Cell Type
Pluripotent Stem Cells
Species
Human, Mouse, Rat
Application
Genome Editing
Area of Interest
Disease Modeling, Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
76026
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
76026
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
76026
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
76026
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more