EasySep™ RBC Depletion Reagent
Immunomagnetic negative selection
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EasySep™ RBC Depletion Reagent -
EasySep™ RBC Depletion Reagent for RoboSep™ -
Label for EasySep™ RBC Depletion Reagent -
Label for EasySep™ RBC Depletion Reagent for RoboSep™
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Overview
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.
This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
Advantages:
The benefits of this kit include:
• 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation or lysis
• Fast, easy-to-use and column-free
• Isolated cells are untouched
• 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation or lysis
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components:
- EasySep™ RBC Depletion Reagent (Catalog #18170)
- EasySep™ RBC Depletion Reagent, 10 mL
- EasySep™ RBC Depletion Reagent for RoboSep™ (Catalog #18170RF)
- EasySep™ RBC Depletion Reagent for RoboSep™, 4 x 2.5 mL
- RoboSep™ Buffer (Catalog #20104) x 2
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Species:
Human
Sample Source:
Whole Blood
Selection Method:
Negative
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; Drug Discovery and Toxicity Testing; HLA; Immunology
Related Products
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Reagent for lysis of red blood cells -
EasySep™ Dead Cell Removal (Annexin V) Kit
Immunomagnetic depletion cell isolation kit -
Density gradient medium for the isolation of mononuclear cells -
For depletion of red blood cells from fresh blood samples and isolation of nucleated cells
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Data
Figure 1. EasySep™ RBC Depletion Reagent Provides Superior RBC Depletion Compared to Ammonium Chloride Lysis
Different types of RBC-containing samples from normal healthy donors were processed to remove RBCs by using either ammonium chloride (NH4Cl) lysis or immunomagnetic depletion with EasySep™ RBC Depletion Reagent. After RBC removal, samples were stained with fluorochrome-conjugated anti-CD45 and anti-Glycophorin A antibodies and analyzed by flow cytometry. Residual RBCs were identified as Glycophorin A+/CD45− events. (A) The percentages of residual RBCs in various samples following use of EasySep™ RBC Depletion Reagent was significantly lower compared to samples treated with ammonium chloride (mean ± SD; n = 31). (B) Both, RBC lysis with ammonium chloride and RBC removal using EasySep™ RBC Depletion Reagent resulted in an equivalent total number of white blood cells recovered from whole blood samples (mean ± SD; n = 37).
Figure 2. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Human Whole Blood Samples
Starting with human whole blood from normal healthy donors, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 2 ± 3 (mean ± SD; n = 31). In the above example, the residual RBC content is 0.05%.
Figure 3. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Human Bone Marrow Samples
Starting with human bone marrow, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 0.39 ± 0.34 (mean ± SD; n = 5). In the above example, the residual RBC content is 0.57%.
Figure 4. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Buffy Coat Samples
Starting with buffy coat, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 0.12 ± 0.08 (mean ± SD; n = 6). In the above example, the residual RBC content is 0.26%.
Figure 5. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Cord Blood Samples
Starting with cord blood, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 1.7 ± 2.2 (mean ± SD; n = 16). In the above example, the residual RBC content is 0.08%.
Figure 6. Typical RBC Removal Using EasySep™ RBC Depletion Reagent With Leukapheresis Samples
Starting with leukapheresis samples, the percentage of residual RBCs (Glycophorin A+/CD45-) following use of EasySep™ RBC Depletion Reagent is typically 0.5 ± 0.8 (mean ± SD; n = 15). In the above example, the residual RBC content is 0.6%.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.