EasySep™ Dead Cell Removal (Annexin V) Kit

Immunomagnetic depletion cell isolation kit

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EasySep™ Dead Cell Removal (Annexin V) Kit

Immunomagnetic depletion of apoptotic (Annexin V+) cells from culture or tissue preparations

1 x 109 cells
Catalog #17899
386 USD

Required Products

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Dead Cell Removal (Annexin V) kit is designed to deplete apoptotic (Annexin V+) cells from cell culture or tissue preparations by immunomagnetic negative selection. Unwanted cells are labeled with Annexin V, antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are simply poured off into a new tube. Annexin V binds phosphatidylserine on the outer leaflet of the cell membrane during apoptosis.
Advantages:
• Fast and easy-to-use
• No columns required
• Compatible across EasySep™, "The Big Easy", and EasyEights™ platforms
Components:
  • EasySep™ Dead Cell Removal (Annexin V) Kit (Catalog #17899)
    • EasySep™ Dead Cell Removal (Annexin V) Cocktail, 0.5 mL
    • EasySep ™ Biotin Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
 
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000), or
• “The Big Easy” EasySep™ Magnet (Catalog #18001), or
• EasyEights™ EasySep™ Magnet (Catalog #18103)
 
Subtype:
Cell Isolation Kits
Cell Type:
Lymphocytes
Species:
Human; Mouse; Rat; Non-Human Primate; Other
Sample Source:
Cord Blood; Leukapheresis; Lung; Lymph Node; Other; Spleen
Selection Method:
Depletion
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(6)
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Video
5:51
How to Perform Cell Counts with a Hemocytometer
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Scientific Poster
Depletion of Dead Cells from Primary Tissue in 6 Minutes

Data and Publications

Data

Starting with human polymorphonuclear cells (PMNCs) cultured overnight, the live cell content (AnnexinV-/PI-) of the enriched fraction is typically 69.7± 12.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the percentages of live cells in the start and final enriched fractions are 12.8% and 74.9%, respectively.

Starting with 24- to 48-hour-old mouse splenocytes, the live cell content of the enriched fraction is typically 79.8 ± 11.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the percentages of live cells in the start and final enriched fractions are 78.1% and 93.4%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.