NeuroCult™ Proliferation Supplement (Mouse & Rat)
Supplement for expansion of mouse and rat neural stem and progenitor cells
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Overview
NOTE: When preparing Complete NeuroCult™ Proliferation Medium, addition of Human Recombinant EGF (Catalog #78006.1) is required. When culturing cells obtained from adult mouse or rat, Human Recombinant bFGF (Catalog #78003.1) and Heparin Solution (Catalog #07980) are also required.
Data Figures

Figure 1. Comparison of Cell Expansion for Mouse Neurospheres Cultured with Complete NeuroCult™ Proliferation Medium (Mouse & Rat) or a Traditional Formulation
Cells microdissected from the cortices of E14 mice were cultured in Complete NeuroCult™ Proliferation Medium (Mouse & Rat) or a traditional formulation containing 20 ng/mL rh EGF. At Day 71, cells cultured in Complete NeuroCult™ Proliferation Medium (Mouse & Rat) were at Passage 13 while cells cultured in a traditional medium formulation were at Passage 10. Complete NeuroCult™ Proliferation Medium (Mouse & Rat) consists of NeuroCult™ NSC Basal Medium (Mouse & Rat), NeuroCult™ NSC Proliferation Supplement (Mouse & Rat) and 20 ng/mL rh EGF.

Figure 2. Cell Expansion for Rat Neurospheres Cultured with Complete NeuroCult™ Proliferation Medium (Mouse & Rat)
Cells microdissected from the cortices of E18 rat were cultured in 3 different lots of NeuroCult™ Proliferation Medium (Mouse & Rat). In each sample, cells continued to generate neurospheres beyond passage 5, resulting in an increase in total cell number. At passages 1 and 5, cells dissociated from the neurospheres were able to differentiate into neurons, oligodendrocytes and astrocytes [data not shown].
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Educational Materials (8)
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Abstract
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Legal Statement:
Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.