PneumaCult™-ALI Medium
Overview
Together, PneumaCult™-ALI Medium and PneumaCult™-Ex Plus Medium (Catalog #05040) constitute a fully integrated BPE-free culture system for in vitro human airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.
Learn how to culture human airway epithelial cells at the ALI in our On-Demand Pulmonary Course or browse our Frequently Asked Questions (FAQs) about the ALI culture workflow using PneumaCult™.
• PneumaCult™-ALI is serum-free and BPE-free to minimize variability
- PneumaCult™-ALI Basal Medium, 450 mL
- PneumaCult™-ALI 10X Supplement, 50 mL
- PneumaCult™-ALI Maintenance Supplement (100X), 5 x 1 mL
Scientific Resources
Product Documentation
Document Type | Product Name | Catalog # | Lot # | Language |
---|---|---|---|---|
Document Type
Product Information Sheet
|
Product Name
PneumaCult™-ALI Medium with 6.5 mm Transwell® Inserts
|
Catalog #
05022 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet
|
Product Name
PneumaCult™-ALI Medium with 12 mm Transwell® Inserts
|
Catalog #
05021 |
Lot #
All |
Language
English |
Document Type
Product Information Sheet
|
Product Name
PneumaCult™-ALI Medium
|
Catalog #
05001 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
PneumaCult™-ALI Medium with 6.5 mm Transwell® Inserts
|
Catalog #
05022 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
PneumaCult™-ALI Medium with 6.5 mm Transwell® Inserts
|
Catalog #
05022 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 3
|
Product Name
PneumaCult™-ALI Medium with 6.5 mm Transwell® Inserts
|
Catalog #
05022 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
PneumaCult™-ALI Medium with 12 mm Transwell® Inserts
|
Catalog #
05021 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
PneumaCult™-ALI Medium with 12 mm Transwell® Inserts
|
Catalog #
05021 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 3
|
Product Name
PneumaCult™-ALI Medium with 12 mm Transwell® Inserts
|
Catalog #
05021 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 1
|
Product Name
PneumaCult™-ALI Medium
|
Catalog #
05001 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 2
|
Product Name
PneumaCult™-ALI Medium
|
Catalog #
05001 |
Lot #
All |
Language
English |
Document Type
Safety Data Sheet 3
|
Product Name
PneumaCult™-ALI Medium
|
Catalog #
05001 |
Lot #
All |
Language
English |
Educational Materials (18)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Overview of the PneumaCult™ Culture System
Expansion of human bronchial epithelial cells (HBECs) in submerged culture is performed with PneumaCult™-Ex Plus or PneumaCult™-Ex. During the early Expansion Phase of the ALI culture procedure, PneumaCult™-Ex Plus or PneumaCult™-Ex is applied to the apical and basal chambers. Upon reaching confluence, the culture is air-lifted by removing the culture medium from both chambers, and adding PneumaCult™-ALI to the basal chamber only. Differentiation into a pseudostratified mucociliary epithelium is obtained following 21-28 days of incubation and can be maintained for more than one year.

Figure 2. HBECs Cultured in PneumaCult™-Ex Successfully Differentiate into a Pseudostratified Mucociliary Epithelium with PneumaCult™-ALI
Early-passage (P1-3) HBECs cultured in PneumaCult™-Ex successfully differentiate when cultured at air-liquid interface with PneumaCult™-ALI for 28 days. H&E staining revealed the pseudostratifi ed structure of the epithelium with cilia present at the apical surface (A). Periodic acid-Schiff staining demonstrated the presence of goblet cells (B). The presence of ciliated and goblet cells was also demonstrated by immunofl uorescence staining of cilia marker acetylated (AC)-Tubulin (green; C) and the goblet cell marker Mucin5AC (green; D). Appropriate positioning of basal cells along the transwell insert was visualized by immunofl uorescence staining using the basal cell markers p75NTR (green) and p63 (red; E,F). A representative merged image indicates the apical cells, detected by DAPI alone, positioned along the epithelium and in close contact with the basal cells (detected by DAPI, p63 and p75NTR co-labeling) located along the insert (G).

Figure 3. Electrophysiological characterization of differentiated HBECs (P4) that were expanded in PneumaCult™-Ex Plus, PneumaCult™-Ex, and Bronchial Epithelial Growth Media
TEER (A) and representative characterization of the ion channel activities (B) for ALI cultures at 28 days post air-lift using HBECs expanded in PneumaCult™-Ex Plus, PneumaCult™-Ex, or Bronchial Epithelial Growth Media. Amiloride: ENaC inhibitor. IBMX and Forskolin: CFTR activators. Genistein: CFTR potentiator. CFTRinh-172: CFTR inhibitor. UTP: Calciumactivated Chloride channels (CaCCs) activator. All ALI differentiation cultures were performed using PneumaCult™-ALI.
Publications (98)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.