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Register: On-Demand Pulmonary Course

Register now to complete an on-demand course on culturing human airway epithelial cells (HAECs) at the air-liquid interface (ALI).

In this self-paced, virtual course, you will learn how to grow human airway epithelial cells (HAECs) at the air-liquid interface (ALI) through a series of lectures, technical lab videos, and assessments.

Instructor Bios

Tyler Brown, Senior Research Associate, Pulmonary Group

Dr. Jennifer Brockhausen, Scientific Support Specialist

Dr. Daniela Mora Ortiz, Scientific Support Specialist

Dr. Kiran Bhullar, Scientific Support Specialist

Course Curriculum

Session 1: Human Airway Culture Systems & Their Applications

Lecture 1: Introduction to Human Airway Culture Systems

  • Morphological structure of large vs small airway
  • Airway culture systems
  • Cell quality, function, and characterization

Lecture 2: Applications of Human Airway Culture Systems

Mini Quiz: Test Your Knowledge


Estimated time for completion: 30 minutes

Session 2: Introduction to Air-Liquid Interface Cultures

Technical Video: How to Model the Human Airway at the Air-Liquid Interface

  • Introduction to the ALI culture workflow using PneumaCult™

Mini Quiz: Test Your Knowledge


Estimated time for completion: 10 minutes

Session 3: Expansion of Human Airway Epithelial Cells*

Technical Video: Expansion of HBECs

  • Expansion culture in flasks
  • Plating
  • Morphological assessment
  • Submerged expansion in inserts

Mini Quiz: Test Your Knowledge


Estimated time for completion: 10 minutes

Session 4: Differentiation of Human Airway Epithelial Cells*

Technical Video: Differentiation of HBECs

  • Media preparation
  • Air-lifting the culture
  • Time course of morphological changes in culture
  • Histological staining

Mini Quiz: Test Your Knowledge


Estimated time for completion: 15 minutes

Final Assessment

Complete this final assessment to test your knowledge and receive a certificate following successful completion.


Estimated time for completion: 15 minutes

*Note: In sessions 3 & 4, we use human bronchial epithelial cells (HBECs) as an example. The procedural steps are also applicable for culturing human small airway epithelial cells (HSAECs). For more details, refer to the Product Information Sheet (PIS) for PneumaCult™-ALI-S Medium.

This on-demand course is divided into four sessions and a final assessment. Following completion of the course, trainees can choose to receive a certificate of completion. Note that this is not an accredited course and cannot be used to receive Continuing Education (CE) credits. If you wish to take an accredited course, please see our live virtual training.