Register: On-Demand Pulmonary Course
Register now to complete an on-demand course on culturing human airway epithelial cells (HAECs) at the air-liquid interface (ALI).
In this self-paced, virtual course, you will learn how to grow human airway epithelial cells (HAECs) at the air-liquid interface (ALI) through a series of lectures, technical lab videos, and assessments.
Instructor Bios
Tyler Brown, Senior Research Associate, Pulmonary Group
Dr. Jennifer Brockhausen, Scientific Support Specialist
Dr. Daniela Mora Ortiz, Scientific Support Specialist
Dr. Kiran Bhullar, Scientific Support Specialist
Course Curriculum
Lecture 1: Introduction to Human Airway Culture Systems
- Morphological structure of large vs small airway
- Airway culture systems
- Cell quality, function, and characterization
Lecture 2: Applications of Human Airway Culture Systems
Mini Quiz: Test Your Knowledge
Estimated time for completion: 30 minutes
Technical Video: How to Model the Human Airway at the Air-Liquid Interface
- Introduction to the ALI culture workflow using PneumaCult™
Mini Quiz: Test Your Knowledge
Estimated time for completion: 10 minutes
Technical Video: Expansion of HBECs
- Expansion culture in flasks
- Plating
- Morphological assessment
- Submerged expansion in inserts
Mini Quiz: Test Your Knowledge
Estimated time for completion: 10 minutes
Technical Video: Differentiation of HBECs
- Media preparation
- Air-lifting the culture
- Time course of morphological changes in culture
- Histological staining
Mini Quiz: Test Your Knowledge
Estimated time for completion: 15 minutes
Complete this final assessment to test your knowledge and receive a certificate following successful completion.
Estimated time for completion: 15 minutes
*Note: In sessions 3 & 4, we use human bronchial epithelial cells (HBECs) as an example. The procedural steps are also applicable for culturing human small airway epithelial cells (HSAECs). For more details, refer to the Product Information Sheet (PIS) for PneumaCult™-ALI-S Medium.