mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells
mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells

1 Kit
Catalog # 03950
526 USD
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Required Products
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. 37μm Reversible Strainers|27215,27250
    Reversible Strainers

    Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

  3. Y-27632 (Dihydrochloride)
    Y-27632

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

Overview

mTeSR™3D was developed for expansion and scale-up of undifferentiated human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. The novel fed-batch workflow saves time and media. Daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.

mTeSR™3D is a specialised and serum-free formulation. This culture system is compatible with a range of suspension culture vessels.
Components
  • mTeSR™3D Seed Basal Medium, 400 mL
  • mTeSR™3D Seed 5X Supplement, 100 mL
  • mTeSR™3D Feed Supplement A, 100 mL
  • mTeSR™3D Feed Supplement B, 12 mL
  • Subtype
    Specialized Media
    Cell Type
    Pluripotent Stem Cells
    Species
    Human
    Application
    Cell Culture, Expansion
    Brand
    TeSR
    Area of Interest
    Stem Cell Biology
    Formulation
    Serum-Free

    Scientific Resources

    Product Documentation

    Document Type Product Name Catalog # Lot # Language
    Document Type
    Product Information Sheet
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Manual
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 3
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 4
    Product Name
    mTeSR™3D
    Catalog #
    03950
    Lot #
    All
    Language
    English

    Educational Materials (8)

    Brochure
    Products for Human Pluripotent Stem Cells
    Brochure
    mTeSR™3D Suspension Culture Medium for hPSCs
    Brochure
    qPCR Arrays for Cell Characterization
    Brochure
    Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
    Technical Bulletin
    mTeSR™3D: Clump Passaging Method for Suspension Culture hPSCs
    Video
    Large-Scale Expansion of Human Pluripotent Stem Cells in 3D Suspension Culture with mTeSR™3D Medium
    1:58
    Large-Scale Expansion of Human Pluripotent Stem Cells in 3D Suspension Culture with mTeSR™3D Medium
    Video
    How to Passage hPSC Aggregates in mTeSR™3D Suspension Culture with a Clump Passaging Method
    5:14
    How to Passage hPSC Aggregates in mTeSR™3D Suspension Culture with a Clump Passaging Method
    Webinar
    mTeSR™3D for Expansion and Scale Up of Human Pluripotent Stem Cell Cultures
    28:08
    mTeSR™3D for Expansion and Scale Up of Human Pluripotent Stem Cell Cultures

    Product Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Data and Publications

    Data

    Figure 1. hPSC growth in mTeSR™3D

    mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).

    Figure 2. hPSC aggregate morphology in mTeSR3D

    Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.


    Figure 3. Expression of undifferentiated cell markers

    hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.

    Figure 4. Tri-lineage Differentiation

    hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).

    Contact STEMCELL Technologies

    Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
    •  

    StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.