mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells

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mTeSR™3D

Serum-free media for suspension culture of human ES and iPS cells

1 Kit
Catalog #03950
486 USD

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Overview

mTeSR™3D was developed for expansion and scale-up of undifferentiated human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. The novel fed-batch workflow saves time and media. Daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.

mTeSR™3D is a specialised and serum-free formulation. This culture system is compatible with a range of suspension culture vessels.
Components:
  • mTeSR™3D Seed Basal Medium, 400 mL
  • mTeSR™3D Seed 5X Supplement, 100 mL
  • mTeSR™3D Feed Supplement A, 100 mL
  • mTeSR™3D Feed Supplement B, 12 mL
  • Subtype:
    Specialized Media
    Cell Type:
    Pluripotent Stem Cells
    Species:
    Human
    Application:
    Expansion; Cell Culture
    Brand:
    TeSR
    Area of Interest:
    Stem Cell Biology
    Formulation:
    Serum-Free

    Scientific Resources

    Educational Materials

    (8)

    Product Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Data and Publications

    Data

    Figure 1. hPSC growth in mTeSR™3D

    mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).

    Figure 2. hPSC aggregate morphology in mTeSR3D

    Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.


    Figure 3. Expression of undifferentiated cell markers

    hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.

    Figure 4. Tri-lineage Differentiation

    hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).

    STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.