This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
(A) The biological activity of Mouse Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Cell viability was measured after 19 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is 0.013 0.02 ng/mL. (B) 1 μg of Mouse Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant TNF-alpha has a predicted molecular mass of 17.4 kDa.