Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen

Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen

From: 452 USD
Catalog #
70060_C
Primary human cells, frozen

Overview

Streamline your assays with ready-to-use, ethically sourced, mobilized primary CD34+ cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.

Donors are mobilized with:
• G-CSF: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70060)
• Plerixafor: a maximum of 0.24 mg/kg plerixafore for 1 day prior to collection (Catalog #70075)
• G-CSF and Plerixafor: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70073)

Isolated from leukapheresis samples using positive immunomagnetic separation techniques and cryopreserved in serum-free medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains
• Serum-free cryopreservation medium
• 10% dimethyl sulfoxide (DMSO)
Subtype
Frozen
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Donor Status
Normal
Purity
≥ 90% CD34+ (as a percentage of CD45+ cells) by flow cytometry

Data Figures

Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells Generate Hematopoietic Colonies in CFU Assays

Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells (Catalog #70060) were thawed, plated at a concentration of 5 x 10^2 viable cells/dish and cultured in MethoCult™ Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells in the sample, imaged with STEMvision™.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
70060, 70060.1, 70060.2, 70060.3, 70060.4
Lot #
All
Language
English
Catalog #
70060, 70060.1, 70060.2, 70060.3, 70060.4
Lot #
All
Language
English
Catalog #
70073, 70073.1, 70073.2, 70073.3, 70073.4
Lot #
All
Language
English
Catalog #
70073, 70073.1, 70073.2, 70073.3, 70073.4
Lot #
All
Language
English
Catalog #
70075, 70075.1, 70075.2, 70075.3, 70075.4
Lot #
All
Language
English
Catalog #
70075, 70075.1, 70075.2, 70075.3, 70075.4
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

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