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Data and Publications
(A) The biological activity of Human Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the
presence of actinomycin D. Cell viability was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined
as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is
(B) 1 μg of Human Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant TNF-alpha has a predicted molecular mass of 17.5 kDa.