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Human Recombinant TNF-alpha

Tumor necrosis factor alpha

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From: 78 USD

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Tumor necrosis factor alpha
From: 78 USD

Overview

Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-kB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immuno- suppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová & Hosek). Other effects of TNF-α include vasodilatation and edema formation.
Alternative Names:
Cachectin, Cachexin, Cytotoxin, DIF, Necrosin, TNF, TNF-α, TNFSF2, Tumor necrosis factor-α
Cell Type:
Airway Cells; Intestinal Cells; Hematopoietic Stem and Progenitor Cells; Neurons; B Cells; NK Cells
Species:
Human
Area of Interest:
Immunology; Stem Cell Biology; Epithelial Cell Biology; Neuroscience
Molecular Weight:
17.5 kDa
Purity:
≥ 95%

Scientific Resources

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Educational Materials

(3)

Data and Publications

Data

(A) The biological activity of Human Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Cell viability was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is 0.4 0.6 ng/mL.
(B) 1 μg of Human Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant TNF-alpha has a predicted molecular mass of 17.5 kDa.

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