Human Recombinant GM-CSF, ACF

Granulocyte-macrophage colony-stimulating factor, animal component-free
Catalog #
78140_C
Granulocyte-macrophage colony-stimulating factor, animal component-free
From: 90 USD

Overview

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitors (Barreda et al.). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils and basophils) and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Monocytes, Myeloid Cells
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
14.6 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant GM-CSF, ACF
Catalog #
78140, 78140.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant GM-CSF, ACF
Catalog #
78140, 78140.1
Lot #
All
Language
English

Educational Materials (8)

Brochure
Tools For Your Immunology Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
Webinar
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
1:06:52
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Product Applications

Data and Publications

Data

(A) The biological activity of Human Recombinant GM-CSF, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 62 pg/mL. (B) 1 μg of Human Recombinant GM-CSF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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