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EasySep™ Human T Cell Isolation Kit

8-Minute cell isolation kit using immunomagnetic negative selection

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human T Cell Isolation Kit

8-Minute cell isolation kit using immunomagnetic negative selection

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8-Minute cell isolation kit using immunomagnetic negative selection
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Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 98% purity with high recovery

  • Untouched, viable cells

What's Included

  • EasySep™ Human T Cell Isolation Kit (Catalog #17951)
    • EasySep™ Human T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • EasySep™ Human T Cell Isolation Kit (Catalog #100-0695)
    • EasySep™ Human T Cell Isolation Cocktail, 1 x 10 mL
    • EasySep™ Dextran RapidSpheres™, 1 x 10 mL
  • RoboSep™ Human T Cell Isolation Kit (Catalog #17951RF)
    • EasySep™ Human T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

What Our Scientist Says

Isolating T cells doesn't have to take a long time. We developed this 8-minute T cell isolation kit so you can get to your downstream experiments sooner.

Neil MacDonaldTechnical Scientist
Neil MacDonald, Technical Scientist

Overview

The EasySep™ Human T Cell Isolation Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This product can be used in place of the EasySep™ Human T Cell Enrichment Kit (Catalog #19051) for even faster cell isolations.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyPlate™ EasySep™ Magnet (Catalog 18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
• Easy 250 EasySep™ Magnet (Catalog #100-0821)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology, Cell Therapy Development

Data Figures

T Cell Separation using EasySep™ Human T Cell Isolation Kit

Figure 1. EasySep™ Human T Cell Isolation Kit

Starting with human peripheral blood mononuclear cells (PBMCs), the T cell content (CD3+) of the isolated fraction is typically 96.7 ± 1.5% (mean ± SD).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human T Cell Isolation Kit
Catalog #
17951RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human T Cell Isolation Kit
Catalog #
17951
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human T Cell Isolation Kit
Catalog #
100-0695
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human T Cell Isolation Kit
Catalog #
17951RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human T Cell Isolation Kit
Catalog #
17951RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human T Cell Isolation Kit
Catalog #
17951RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human T Cell Isolation Kit
Catalog #
17951
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human T Cell Isolation Kit
Catalog #
17951
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human T Cell Isolation Kit
Catalog #
100-0695
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human T Cell Isolation Kit
Catalog #
100-0695
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (25)

T Cell Reagents for Your Cellular Therapy Research
Brochure
T Cell Reagents for Your Cellular Therapy Research
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Isolate Human Immune Cells
Brochure
Isolate Human Immune Cells
Human T Cell Research Product Workflow
Brochure
Human T Cell Research Product Workflow
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Production of Chimeric Antigen Receptor T Cells
Wallchart
Production of Chimeric Antigen Receptor T Cells
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
How to Isolate Cells in 96-Well Plates Using the EasyPlate™ Cell Separation Magnet
2:34
Video
How to Isolate Cells in 96-Well Plates Using the EasyPlate™ Cell Separation Magnet
EasySep™のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep™のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Non-Viral CRISPR Knock-In Anti-B7-H3 CAR-T Cells Are Amenable for Treatment of Subtypes of Small Cell Lung Cancer
26:48
Webinar
Non-Viral CRISPR Knock-In Anti-B7-H3 CAR-T Cells Are Amenable for Treatment of Subtypes of Small Cel...
Next-Generation Tools to Advance Your Human T Cell Research
32:50
Webinar
Next-Generation Tools to Advance Your Human T Cell Research
Accelerating T Cell Therapy Research
Webinar
Accelerating T Cell Therapy Research
T Cell Differentiation and Cancer Immunity
48:32
Webinar
T Cell Differentiation and Cancer Immunity
Next-Generation Cell Isolation Solutions for T Cell Therapy Research
27:28
Webinar
Next-Generation Cell Isolation Solutions for T Cell Therapy Research
How to Optimize Your T Cell Therapy Workflow—Without the Use of Serum or Feeder Cells
27:26
Webinar
How to Optimize Your T Cell Therapy Workflow—Without the Use of Serum or Feeder Cells
How to Create CRISPR-Edited T Cells
36:25
Webinar
How to Create CRISPR-Edited T Cells
Online Immunology Journal Club: Human In Vitro T Cell Development
31:22
Webinar
Online Immunology Journal Club: Human In Vitro T Cell Development
Workflow Solutions for Human T Cell Isolation and Expansion
Scientific Poster
Workflow Solutions for Human T Cell Isolation and Expansion

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Publications (11)

Inflammation-Induced Mucosal KYNU Expression Identifies Human Ileal Crohn's Disease. M. Huhn et al. Journal of clinical medicine 2020 may

Abstract

The widely varying therapeutic response of patients with inflammatory bowel disease (IBD) continues to raise questions regarding the unclarified heterogeneity of pathological mechanisms promoting disease progression. While biomarkers for the differentiation of Crohn's disease (CD) versus ulcerative colitis (UC) have been suggested, specific markers for a CD subclassification in ileal CD versus colonic CD are still rare. Since an altered signature of the tryptophan metabolism is associated with chronic inflammatory disease, we sought to characterize potential biomarkers by focusing on the downstream enzymes and metabolites of kynurenine metabolism. Using immunohistochemical stainings, we analyzed and compared the mucosal tryptophan immune metabolism in bioptic samples from patients with active inflammation due to UC or CD versus healthy controls. Localization-specific quantification of immune cell infiltration, tryptophan-metabolizing enzyme expression and mucosal tryptophan downstream metabolite levels was performed. We found generally increased immune cell infiltrates in the tissue of all patients with IBD. However, in patients with CD, significant differences were found between regulatory T cell and neutrophil granulocyte infiltration in the ileum compared with the colon. Furthermore, we observed decreased kynurenine levels as well as strong kynureninase (KYNU) expression specifically in patients with ileal CD. Correspondingly, significantly elevated levels of the kynurenine metabolite 3-hydroxyanthranilic acid were detected in the ileal CD samples. Highlighting the heterogeneity of the different phenotypes of CD, we identified KYNU as a potential mucosal biomarker allowing the localization-specific differentiation of ileal CD versus colonic CD.
View publication
An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. E. V. Vinogradova et al. Cell 2020 aug

Abstract

Electrophilic compounds originating from nature or chemical synthesis have profound effects on immune cells. These compounds are thought to act by cysteine modification to alter the functions of immune-relevant proteins; however, our understanding of electrophile-sensitive cysteines in the human immune proteome remains limited. Here, we present a global map of cysteines in primary human T cells that are susceptible to covalent modification by electrophilic small molecules. More than 3,000 covalently liganded cysteines were found on functionally and structurally diverse proteins, including many that play fundamental roles in immunology. We further show that electrophilic compounds can impair T cell activation by distinct mechanisms involving the direct functional perturbation and/or degradation of proteins. Our findings reveal a rich content of ligandable cysteines in human T cells and point to electrophilic small molecules as a fertile source for chemical probes and ultimately therapeutics that modulate immunological processes and their associated disorders.
View publication
Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures. M. Reyes et al. Science advances 2019 jan

Abstract

Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system's technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings.
View publication
View All Publications

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