Human Peripheral Blood Pan-T Cells, Frozen
Primary human cells, frozen
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Overview
Isolated from peripheral blood mononuclear cells using negative immunomagnetic separation and cryopreserved in animal component-free CryoStor®CS10 medium (Catalog #07930), pan T cells consist of CD4 and CD8 T cells and some gamma/delta T cell subsets. Cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.
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Data Figures

Figure 1. Cryopreserved Pan-T Cells Secrete IL-2 Upon Activation
T cells freshly isolated from a Leukopak (Catalog #70500) using EasySep™ Human T Cell Isolation Kit (Catalog #17951) or cryopreserved Pan-T Cells (Catalog #70024) were cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) and incubated for 48 hours with or without ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971). Freshly isolated and cryopreserved purified T cells secrete similar levels of IL-2 upon activation as measured using the Human IL-2 ELISA Kit (Catalog #02006). *IL-2 concentration of control in culture was lower than the limit of detection.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
Educational Materials (7)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.