IntestiCult™ organoid culture media are complete growth media that support establishment, expansion, long-term maintenance, and further differentiation of intestinal organoid cultures from human or
intestinal crypts. Developed in collaboration with Dr. Hans Clevers and Foundation Hubrecht Organoid Technology (The HUB) this complete and defined medium generates organoids from intestinal crypts in less than a week. These ‘mini-guts’ retain the crypt- and villus-like domains, a central lumen and all major cell types found in the adult intestinal epithelium. The organoids are functional and ready for use in a number of research applications including disease modeling, drug screening and tissue regeneration.
Studying the intestinal epithelium can pose multiple challenges. Traditional in vitro monolayer cultures are convenient but lack key structural features and the cellular diversity of an adult intestine. In vivo animal models allow experimentation on an intact intestine but are often more difficult and expensive to run, and provide limited relevance to human physiology. Intestinal organoids address many of these issues by providing a convenient in vitro system that has high physiological relevance.
What are Intestinal Organoids?
Intestinal organoids are three-dimensional multicellular structures that retain key features of the adult intestinal epithelium, such as the crypt- and villus-like domains, a central lumen and the major cell types: intestinal stem cells, Paneth cells, goblet cells, enteroendocrine cells and enterocytes. Organoid culture is a convenient and physiologically relevant tool that can be used in a variety of research applications.
We are pleased that STEMCELL will be our partner in making specialty media for growth of organoids available to the scientific community. The broad availability of off-the-shelf cell culture media from a world leader in the development of specialized cell culture media and cell separation products represents an essential step in the further implementation of this exciting technology.
Dr. Hans Clevers, Founding Director of The HUB
Explore our resources to learn more about organoids, and learn how to generate, culture, and differentiate intestinal organoids.