Complete, Defined Medium for Intestinal Organoid Culture
We are pleased that STEMCELL will be our partner in making specialty media for growth of organoids available to the scientific community. The broad availability of off-the-shelf cell culture media from a world leader in the development of specialized cell culture media and cell separation products represents an essential step in the further implementation of this exciting technology.
Dr. Hans Clevers, Founding Director of The HUB
Addressing the Challenges in Intestinal Research
Studying the intestinal epithelium can pose multiple challenges. In vitro monolayer cultures are convenient but lack key structural features and the cellular diversity of an adult intestine. In vivo animal models allow experimentation on an intact intestine but are often more difficult and expensive to run. Intestinal organoids, or ‘mini-guts’, address many of these issues by providing a convenient in vitro system that has high physiological relevance. In this video Dr. Ryan Conder discusses the research potential of this culture system.
What are Intestinal Organoids?
Intestinal organoids are three-dimensional multicellular structures that retain key features of the adult intestinal epithelium, such as the crypt- and villus-like domains, a central lumen and all of the major cell types: intestinal stem cells, Paneth cells, goblet cells, enteroendocrine cells and enterocytes. Organoid culture is a convenient and physiologically relevant tool that can be used in a variety of research applications. Watch this video to learn how you can easily incorporate organoids into your research.
Why Use IntestiCult™?
- A defined medium that does not require additional growth factors.
- No need for expensive cytokines or labor intensive conditioned medium.
- Efficient and reproducible generation of organoids in less than one week.
- Organoids retain key features and all major cell types found in the adult intestinal epithelium.
Products for Intestinal Organoid Culture
Brand HistoryDr. Hans Clevers and his research team have made significant contributions to the field of stem cells and organoid culture. In 2007, Dr. Nick Barker et al. identified the presence of LGR5+ stem cells in the intestinal crypt. In 2009, Dr. Toshiro Sato et al. published a protocol for establishing organoid structures from intestinal crypts or single intestinal stem cells. The protocol described the culture conditions that would support long-term expansion of these organoids without requiring a mesenchymal niche. In 2014, Dr. Clevers and The HUB foundation for Organoid Technology signed an agreement with STEMCELL Technologies to manufacture and distribute cell culture media for organoids. In 2015, IntestiCult™ Organoid Growth Medium (Mouse) was released to provide researchers with a convenient, complete and affordable medium for establishing organoid cultures.
Key Applications of Intestinal Organoids
Epithelial Cell Biology
Intestinal Stem Cell Niche
Gene Expression and Function
Infection and Immunity
Wilson SS et al. (2014) A small intestinal organoid model of non-invasive enteric pathogen-epithelial cell interactions. Mucosal Immunol 8(2): 352-61.
Zhang YG et al. (2014) Salmonella-infected crypt-derived intestinal organoid culture system for host-bacterial interactions. Physiol Rep 2(9): e12147.
Transplantation and Engraftment
Schwank G et al. (2013) Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Cell Stem Cell 13(6): 653-8.