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The RPA-T8 antibody reacts with CD8a, a 32 - 34 kDa type I transmembrane glycoprotein which is a subunit of CD8. CD8 is a disulfide-bonded dimer, found either as a heterodimer of CD8a (α) and CD8b (β) subunits (i.e., αβ) or a homodimer (αα). CD8 acts as a co-receptor to the T cell receptor (TCR) during T cell activation by binding MHC Class I molecules presented by an antigen-presenting cell. It functions to strengthen the association between the TCR and MHC I-antigen complex, and to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. The CD8a chain binds to the α3 domain of class I MHC molecules. CD8 is expressed by a majority of thymocytes, a subset of mature peripheral blood T cells (T cytotoxic cells), a proportion of β T cells, and at lower levels by NK cells (which predominantly express CD8a homodimers).
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Direct Human CD8+ T Cell Isolation Kit (Catalog #19663), EasySep™ Human CD8+ T Cell Enrichment Kit (Catalog #19053), EasySep™ Human CD8 Positive Selection Kit (Catalog #18053), EasySep™ Human CD3 Positive Selection Kit (Catalog #18051), EasySep™ HLA CD2 Positive Selection Kit (Catalog #18657HLA), and EasySep™ HLA T Cell Enrichment Kit (Catalog #19051HLA).
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC and Anti-Human CD3 Antibody, Clone UCHT1, FITC (Catalog #60011FI). (B) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC and Anti-Human CD45 Antibody, Clone HI30, FITC (Catalog #60018FI). (C) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC and a mouse IgG1, kappa FITC isotype control antibody. (D) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA T Cell Enrichment Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
Figure 2. Data for Biotin-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
Figure 3. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD3 Positive Selection Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).
Figure 4. Data for PE-Conjugated
(A) Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA T Cell Enrichment Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
Figure 5. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 6. Data for PerCP-Cy55-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD8 Positive Selection Kit and labeled with Anti-Human CD8a Antibody, Clone RPA-T8, PerCP-Cy5.5. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PerCP-Cy5.5 isotype control antibody is shown (solid line histogram).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
Mouse monoclonal IgG1, kappa isotype control antibody
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Anti-Human CD8a Antibody, Clone RPA-T8
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
