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Anti-Human CD2 Antibody, Clone RPA-2.10

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2

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Anti-Human CD2 Antibody, Clone RPA-2.10

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2

From: 109 USD
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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2
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Overview

The RPA-2.10 antibody reacts with CD2, an ~50 kDa type I transmembrane glycoprotein and a member of the immunoglobulin (Ig) superfamily; two Ig-like domains are located in its extracellular portion. CD2 is broadly expressed on peripheral T and NK cells, dendritic cells, erythrocytes, most thymic T cells, subsets of thymic B cells, and on the endothelium. CD2 expression appears early during T cell differentiation. Aberrant expression has been observed in some lymphomas and myeloid leukemias. CD2 is critically important for T cell activation and signaling, as well as lymphocyte adhesion. The primary ligand for CD2 is CD58 (LFA-3) located on antigen-presenting cells, with additional ligands comprising CD15 (SSEA-1), CD48 and CD59. Notably, the RPA-2.10 antibody blocks the mixed lymphocyte reaction.
Subtype
Primary Antibodies
Target Antigen
CD2
Alternative Names
LFA-2, SRBC-R, T11
Reactive Species
Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Human, Pig, Pigtailed Macaque, Rhesus, Squirrel Monkey
Conjugation
APC, FITC, PE, PerCP
Host Species
Mouse
Cell Type
T Cells
Species
Human, Non-Human Primate, Other
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blotting
Area of Interest
Immunology
Clone
RPA-2.10
Gene ID
914
Isotype
IgG1, kappa

Data Figures

Data for Anti-Human CD2 Antibody, Clone RPA-2.10, APC

Figure 1. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, APC

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

Data for Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin

Figure 2. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).

Data for Anti-Human CD2 Antibody, Clone RPA-2.10, FITC

Figure 3. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, FITC

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, FITC and anti-human CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).

Data for Anti-Human CD2 Antibody, Clone RPA-2.10, PE

Figure 4. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, PE

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE and anti-human CD45 APC. (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (open histogram).

Data for Anti-Human CD2 Antibody, Clone RPA-2.10

Figure 5. Data for Anti-Human CD2 Antibody, Clone RPA-2.10

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram), or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Image showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP or a mouse IgG1, kappa isotype control antibody, PerCP.

Figure 6. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, PE
Catalog #
100-0431, 60007PE.1, 60007PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin
Catalog #
60007BT.1, 60007BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, APC
Catalog #
60007AZ.1, 100-0432, 60007AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP
Catalog #
100-0430, 100-0429, 100-0428
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, FITC
Catalog #
100-0427, 60007FI, 100-0426
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10
Catalog #
60007
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, PE
Catalog #
100-0431, 60007PE.1, 60007PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin
Catalog #
60007BT.1, 60007BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, APC
Catalog #
60007AZ.1, 100-0432, 60007AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP
Catalog #
100-0430, 100-0429, 100-0428
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10, FITC
Catalog #
100-0427, 60007FI, 100-0426
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD2 Antibody, Clone RPA-2.10
Catalog #
60007
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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