A cellular protein for detection of apoptotic cells
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Annexin V is used along with viability dyes such as 7-AAD (Catalog #75001) or Propidium Iodide (Catalog #75002). The process of PS translocation occurs prior to the loss of membrane integrity. Therefore, as cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently, viability dyes pass into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability dye.
Figure 1. Data for Annexin V, APC
Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD (Catalog #75001).
Figure 2. Data for Annexin V, FITC
Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC Annexin V and 7-AAD.
Figure 3. Data for Annexin V, PE
Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with PE Annexin V and 7-AAD.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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