EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection kit
EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection of CD45+ leukocytes from mouse lung, splenocytes, or other tissues

2 x 10^9 cells
Catalog # 18945
When using the EasyEights™ EasySep™ Magnet, contact us at techsupport@stemcell.com to request an additional vial of EasySep™ Dextran RapidSpheres™ 50100.
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Mouse CD45 Positive Selection Kit is designed to isolate CD45 cells from single-cell suspensions of splenocytes, lung, or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Advantages
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components
  • EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945)
    • EasySep™ Mouse CD45 Positive Selection Kit Component A, 1 mL
    • EasySep™ Mouse CD45 Positive Selection Kit Component B, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Innate Lymphoid Cells, Leukemia/Lymphoma Cells, Lymphocytes, Macrophages, Megakaryocytes, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Plasma, T Cells, T Cells, Other Subsets
Species
Mouse
Sample Source
Lung, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Mouse CD45 Positive Selection Kit
Catalog #
18945
Lot #
Lot 1000079626 and lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Mouse CD45 Positive Selection Kit
Catalog #
18945
Lot #
Lot 1000079627 and above
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse CD45 Positive Selection Kit
Catalog #
18945
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse CD45 Positive Selection Kit
Catalog #
18945
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse CD45 Positive Selection Kit
Catalog #
18945
Lot #
All
Language
English

Educational Materials (10)

Brochure
EasySep™ Cell Separation Technology
Brochure
Mouse Cell Isolation
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen)
2:12
How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen)
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
How to Isolate Cells in 96-Well Plates Using the EasyPlate™ Cell Separation Magnet
2:34
How to Isolate Cells in 96-Well Plates Using the EasyPlate™ Cell Separation Magnet
Scientific Poster
Isolation of Mouse CD45 Positive Leukocytes from Tissues

Data and Publications

Data

FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit

Figure 1. Typical EasySep™ Mouse CD45 Positive Selection Profile

(A) Starting with a naïve mouse lung single-cell suspension, the leukocyte content (CD45+) of the isolated fraction is typically 97.0% ± 1.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions in lung are 53.1% and 94.1%, respectively. (B) Starting with unlysed naïve mouse splenocytes, the leukocyte content (CD45+) of the isolated fraction is typically 97.6 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example using spleen, the purities of the start and final isolated fractions are 31.2% and 96.6%, respectively.

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