EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection kit

When using the EasyEights™ EasySep™ Magnet, contact us at techsupport@stemcell.com to request an additional vial of EasySep™ Dextran RapidSpheres™ 50100.

EasySep™ Mouse CD45 Positive Selection Kit

Immunomagnetic positive selection kit

EasySep™ Mouse CD45 Positive Selection Kit
2 x 10^9 cells
811 USD
Catalog # 18945

Immunomagnetic positive selection of CD45+ leukocytes from mouse lung, splenocytes, or other tissues

Product Advantages

• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Isolated cells are not fluorochrome-labeled

What's Included

  • EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945)
    • EasySep™ Mouse CD45 Positive Selection Kit Component A, 1 mL
    • EasySep™ Mouse CD45 Positive Selection Kit Component B, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
When using the EasyEights™ EasySep™ Magnet, contact us at techsupport@stemcell.com to request an additional vial of EasySep™ Dextran RapidSpheres™ 50100.

Overview

The EasySep™ Mouse CD45 Positive Selection Kit is designed to isolate CD45 cells from single-cell suspensions of splenocytes, lung, or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Innate Lymphoid Cells, Leukemia/Lymphoma Cells, Lymphocytes, Macrophages, Megakaryocytes, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Plasma, T Cells, T Cells, Other Subsets
Species
Mouse
Sample Source
Lung, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
18945
Lot #
Lot 1000079626 and lower
Language
English
Catalog #
18945
Lot #
Lot 1000079627 and above
Language
English
Document Type
Safety Data Sheet 1
Catalog #
18945
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
18945
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
18945
Lot #
All
Language
English

Data and Publications

FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit FACS Profile Results with EasySep™ Mouse CD45 Positive Selection Kit

Figure 1. Typical EasySep™ Mouse CD45 Positive Selection Profile

(A) Starting with a naïve mouse lung single-cell suspension, the leukocyte content (CD45+) of the isolated fraction is typically 97.0% ± 1.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions in lung are 53.1% and 94.1%, respectively. (B) Starting with unlysed naïve mouse splenocytes, the leukocyte content (CD45+) of the isolated fraction is typically 97.6 ± 1.3% (mean ± SD using the purple EasySep™ Magnet). In the above example using spleen, the purities of the start and final isolated fractions are 31.2% and 96.6%, respectively.

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